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1、甲苯萃取-微波消解-原子荧光光谱法测定富硒保健品中有机硒和总硒李四生1董晓根2李家涛1周勤文1李建伟1(1.合肥市疾病预防控制中心,合肥 230022;2.北京市丰台区疾病预防控制中心,北京 100071)摘要目的:建立分离、消解、测定富硒保健品中有机硒和总硒的分析方法。方法:采用甲苯萃取分离样品中的有机硒后,利用HNO3-H2O2消解体系微波消解,原子荧光光谱法测定样品中的有机硒和总硒。结果:在优化工作条件下,硒的检出限为0.088μg/L,相对标准偏差为0.43%~0.53%,线性范围为0~200μg/L,应用该方法检测不同富硒保
2、健品中的总硒含量为0.87~13.04mg/kg,有机硒含量为0.76~8.54mg/kg,总硒加标回收率为95.1%~106.2%。结论:该方法简便、灵敏、重现性好、干扰少,适合富硒保健品中的有机硒和总硒测定。关键词甲苯萃取;微波消解;原子荧光光谱法;富硒保健品;有机硒;总硒Determinationoforganicandtotalseleniumcontentsinselenium-enrichedhealthfoodbymethylbenzeneextraction-microwave-assisteddigestion-at
3、omicfluorescencespectrometryLiSi-sheng1,DongXiao-gen2,LiJia-tao1,ZhouQin-wen1,LiJian-wei1(1·HefeiCentreforDiseaseControlandPrevention,Hefei230022,China;2·FengtaiCentreforDiseaseControlandPrevention,Beijing100071,China)AbstractObjective:Todevelopamethodfortheseparation,d
4、igestionanddeterminationoforganicandtotalseleniuminselenium-enrichedhealthfood·Methods:Theorganicseleniumextractedandseparatedfromselenium-enrichedhealthfoodwasdigestedbyamixtureofconcentratednitricacidandhydrogenperoxide(HNO3-H2O2)inthesealedvessel·Thentheorganicseleni
5、umwassubjectedtotheanalysisbyatomicfluorescencespectrometry(AFS)·Exceptthemethylbenzeneextraction,totalseleniumwasdigestedandmeasuredassameasorganicselenium·Results:Undertheoptimalworkcondition,thedetectionlimitofseleniumbythismethodwas0.088μg/L,thelinearrange0~200μg//L
6、,therelativestandardderivation0.43%~0.53%·Thetotalseleniumcontentof0.87~13.04mg/kgandtheorganicseleniumcontentof0·76~8·54mg/kgweredetectedrespectivelyfromtheseleniumenrichedhealthfoodbythismethodwiththerecoveryrateof95.1%~106.2%fortotalselenium·Conclusion:Thismethodispr
7、ovedtobesimple,rapid,sensitiveaccurateandrepeatable·Soitissuitabletothedeterminationoforganicandtotalseleniuminselenium-enrichedhealthfood·KeywordsMethylbenzeneextraction;Microwave-assisteddigestion;AFS;Selenium-enrichedhealthfood;Organicselenium;Totalselenium硒(Se)是人体必需
8、微量元素,在生命过程中起着重要作用,大量基础研究和临床实践证实:当Se摄入量不足时,可诱发心血管疾病、大骨节病、肿瘤、胰腺纤维化、白内障等疾病[1];硒也是一种准金属元素,其形态常分为无机硒和有机硒,有机硒比无机硒具有较
