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1、产前诊断的方法(Methodsofprenataldiagnosis)YangGuoan,WangHongkun,ChenPeng,GeHua(CentralLaboratoryoftheFirstAffiliatedHospitalofBaotouMedicalCollege,InnerMongolia,014010)[CLCnumber]R714[documentidentificationcode]A[articlenumber]1672-5085(2011)33-0078-02Objectivetoexploretheapplicationprospec
2、tofFISHtechniqueinprenataldiagnosis・Applicationofdomesticprobe(CSP18/CSPX/CSPY/GLP21probeprobegroupandGLP13group)weredetectedin50casesofamnioticfluidcells,soastojudgewhethertheabnormalnumberof13,18,21,fetalXandYchromosome,and50casesofamnioticfluidchromosomecultureresuItswerecompared.
3、Resultsaccordingtotheexperimentalprocedure,50casesofamnioticfluidFISHweredetectedsuccessfully.Comparedwiththeresultsofamnioticfluidkaryotypeanalysis,theaccuracyratewas100%・However,someFISHresultsinasmallnumberofnucleiwithabnormalhybridizationsignals・Conclusioncomparedwithamnioticflui
4、dculture,FISHhastheadvantagesofshorttimecycleandhighaccuracy,andithasimportantapplicationvalue,butitcannotcompletelyreplacetheroleofamnioticfluidcultureinprenataldiagnosis・Prenataldiagnosisofamnioticfluidbyfluorescenceinsituhybridization[Abstract]ObjectiveTostudyapplicationoffluoresc
5、enceinsituhybridization(FISH)technologyinprenataldiagnosis・MethodsApplyingnationalprobe(probegroupCSP18/CSPX/CSPYandprobegroupGLP13/GLP21todetectfiftyamnioticfluidcells),thendecidingwhetherthenumbersofchromosomal13,18,21,XY,arenormal,andcomparingwithconsequenceoffiftyamnioticfluidcel
6、lculture・ResultsAccordingtoexperimentflowsheetstrictly,FiftFISHdetectsaresuccessful,anditsaccuracyrateiswithconsequenceoffifty100%comparedamniotiefluidchromosomalkaryotypeanalysis・ButpartofFISHdetectsappearedfewhybridizationsignal.ConclusionFISHtechnologyhasafewfeaturesincludingshort
7、timecycleandhighaccuracyratecompAredwithamnioticfluidculture・AlthoughFISHhasimportantapplicationvalue,itcant'ttotallydisplaceeffectofmnioticfluidcultureinprenataldiagnosis・[Keywords]fluorescenceinsituhybridizationprenataldiagnosisamnioticfluidSincetheimplementationofthefamilyplanning
8、policyinChin
