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1、StudyonCountingMethodforMicrocystisCellsYingke-ZHOUCollegeofMiningEngineering,HebeiUnitedUniversityTangShan,HeBei,063009,Yingke5168@sohu.comThispaperexpoundstheultrasonicshort-termeffecttotreatmentwaterinordertofindamethodwhichcountsthecellsofMicrocystisinwatermorescientifically.Byexperiment^itis
2、furtherstudyofultrasonicfrequencyandreactiontimeinfluenceontreatmenteffect.Keywords:Microcystis;count;ultrasonicWaterenvironmenteutrophicationcausedbyalgalbloomshasbecomemoreandmoreseriousinourcountry.FreshwaterlakessuchasTaihu1'1,Dianchi12,31andChaohu121everysummerandautumnexplodecyanobactcrialb
3、loom,whichiscapableofgeneratingmicrocystinmicrocystis・Toxicalgalbloomsoccurringnotonlybringseriousharmtoaquaticecosystem
4、31,butalsoalgalloxinslohumanhealthhavingdirectorpotentialharm141,socontrolingalgalbloomandeliminatingtoxinsharmhavebeenbrookingnodelay.Microcystiscellcountmonitoringwillprovide
5、firsthandinformationforalgalwarningandcontrol.Algaldensitycountingmethodisdividedintoindividualcountingandcellcounting・Becauseofthebiologicalindividualdifferences,especiallyinclustersofMicrocystis,individualofMicrocystisvariesverylargly,thebiggercanreachhundredsoreventhousandsoftimesthanthesmalle
6、匚Socellcountingmethodismorescientifictodeterminethealgaegrowth.Butthecellcountingmethodistime-consumingandmorecountingerrorsuses,sousingtheshort-termeffectofultrasoundtobreakuplargeindividualsmakestheindividualofMicrocystisinwaterbecomesmallerandbemoredistributly.1.RADITIONALMICROSCOPYANDULTRASON
7、ICTREATMENTMETHODCOMPARISONExperimentsselectAandBtwocontainingMicrocystiswatersamples,theneachtakesthe3()mLoftwotofour50mLgroundglassbottle,numberA1,A2,B1andB2.Al,Blusetraditionalmicroscopiccounting151eachwith0」mLalgaecountingplatelocount7;A2,B240KHzultrasonictreatmentabout9min,thenmicroscopiccou
8、nting,eachwith0.1mLalgaecountingplatecountof7,theresultsintable1.Table1shows:therelativestandarddeviationofA,Btwosamplesbyconventionalmicroscopyisin40%above,andthedistributionofMicrocystiscellsinwaterisveryuneven,onlyt
