SDS-PAGE凝胶配方表.doc

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1、浓缩胶（5%Acrylamide）各种组分名称1ml2ml3ml4ml5ml6ml8ml10ml双蒸水0.681.42.12.73.44.15.56.830%Acrylamide0.170.380.50.670.831.01.31.71.0MTris-HCl(PH6.8)0.130.250.380.50.630.751.01.2510%过硫酸铵(AP)0.010.020.030.040.050.060.080.110%SDS0.010.020.030.040.050.060.080.1TEMED0.0010.0020.0030

2、.0040.0050.0060.0080.01分离胶(1)6%Gel各种组分名称5ml10ml15ml20ml25ml30ml40ml50ml双蒸水2.65.37.910.613.215.921.226.530%Acrylamide1.02.03.04.05.06.08.010.01.5MTris-HCl(PH8.8)1.32.53.85.06.37.510.012.510%过硫酸铵(AP)0.050.10.150.20.250.30.40.510%SDS0.050.10.150.20.250.30.40.5TEMED0.00

3、40.0080.0120.0160.020.0240.0320.04(2)8%Gel各种组分名称5ml10ml15ml20ml25ml30ml40ml50ml双蒸水2.34.66.99.311.513.918.523.230%Acrylamide1.32.74.05.36.78.010.713.31.5MTris-HCl(PH8.8)1.32.53.85.06.37.510.012.510%过硫酸铵(AP)0.050.10.150.20.250.30.40.510%SDS0.050.10.150.20.250.30.40.5T

4、EMED0.0030.0060.0090.0120.0150.0180.0240.03(3)10%Gel各种组分名称5ml10ml15ml20ml25ml30ml40ml50ml双蒸水1.84.05.97.99.911.915.919.830%Acrylamide1.73.37.96.78.310.013.316.71.5MTris-HCl(PH8.8)1.32.53.85.06.37.510.012.510%过硫酸铵(AP)0.050.10.150.20.250.30.40.510%SDS0.050.10.150.20.25

5、0.30.40.5TEMED0.0020.0040.0060.0080.010.0120.0160.02(4)12%Gel各种组分名称5ml10ml15ml20ml25ml30ml40ml50ml双蒸水1.63.84.96,68.29.913.216.530%Acrylamide2.04.06.08.010.012.016.020.01.5MTris-HCl(PH8.8)1.32.53.85.06.37.510.012.510%过硫酸铵(AP)0.050.10.150.20.250.30.40.510%SDS0.050.10.

6、150.20.250.30.40.5TEMED0.0020.0040.0060.0080.010.0120.0160.02(5)15%Gel各种组分名称5ml10ml15ml20ml25ml30ml40ml50ml双蒸水1.12.33,44.65.76.99.211.530%Acrylamide2.55.07.510.012.515.020.025.01.5MTris-HCl(PH8.8)1.32.53.85.06.37.510.012.510%过硫酸铵(AP)0.050.10.150.20.250.30.40.510%SDS

7、0.050.10.150.20.250.30.40.5TEMED0.0020.0040.0060.0080.010.0120.0160.02注意：10%的AP溶液配方：过硫酸铵取10克加蒸馏水10ml配成10%浓缩胶用5ml15%的分离胶用10ml