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1、FEMSMicrobiologyLetters20(1983)23-2623PublishedbyElsevierSyncytiumproductionbyhumancoronavirus229EgroupvirusesRuthP.ParryandMalcolmR.MacnaughtonDivisionofCommunicableDiseases,ClinicalResearchCentre,Harrow,Middlesex,HA13UJ,U.K.Received4March1983Revisionreceive
2、dandaccepted26May19831.INTRODUCTIONKI,LP,PRandTOwereisolatedintissuecultureattheCommonColdUnit,Salisbury,U.K.,fromTheCoronaviridaeareagroupoflarge,RNA-nasalwashingsofsubjectswithnaturalcolds[6,7].containingenvelopedvirusesthatcauseawideThevirusesweregrowninst
3、ationarymonolayerrangeofdiseasesintheirhosts[1-3].ManyculturesofhumanembryolungcellsoftheMRCcoronaviruseshavebeenreadilyadaptedtogrowthcontinuous(MRCc)line,originallyobtainedfromincelllinesderivedfromtheirhostsorotherDr.A.F.Bradburne[8].Thecellmonolayersinfla
4、tspecies.Thecytopathiceffect(cpe)producedbybottleswereinfectedataninputmultiplicityof0.1coronavirusesininfectedcellsisvariable[1].How-infectiousparticlespercelland,followinganad-ever,themostcommonfeatureofthecpeisitssorptionperiodof1hat33°C,wereincubatedatfoc
5、alnaturewhichprogressestoinvolvetheentire33°Cfor24hinEagle'sBMEmediumwith2%cellsheet.Ontheotherhand,thecpeformedbynewborncalfserum.Afterincubationthecellssomecoronavirusesisofamorenonspecificde-wereharvested,frozenandthawedonce,clarifiedgenerativequality.Most
6、coronavirusesproduceaat2000xgfor30rainat4°C,andthesupernatantcpewithatendencytoformsyncytia,althoughcontaininginfectiousvirusparticleswasstoredatthiscannotatpresentbeconsideredtobeagen--70°Cuntilrequired.eralfeatureoftheCoronaviridae.AcloneoftheMRCcs,calledMR
7、C16,wasUpto30humancoronaviruses(HCVs)haveobtainedfromDr.R.PhillpottsandusedtogetherbeenisolatedandtheyallbelongtooneoftwowithMRCcs,forsyncytialformationstudies.Thedistinctserotypes,namedaftertheirprototypefiveHCV299E-groupvirusesgrewtosimilartitresviruses229E
8、[4]andOC43[5].Inthisstudy,theinbothMRCcsandMRC16s.Cellsinroller-cul-inductionofsyncytiaintissueculturesbyfiveturetubeswereinfectedasdescribedaboveandcloselyrelated229Egroupvirusesisexamin
