冠状病毒Coronavirusl论文-2009 P_062 Human coronavirus NL63 and 229E seroconversion in children.pdf

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1、S30Posterpresentations,ESCV2009,Amsterdam/JournalofClinicalVirology44Suppl.1(2008)S17–S32P.059P.061DetectionofEpstein–BarrVirusDNAinrespiratoryspecimensfrompa-Clinicalevaluationofpediatricviralacuterespiratorytractinfectionstientswithchronicobstructi

2、vepulmonarydiseasebyquantitativePCRdetectedbymultiplexreal-timePCRD.J.Fairley,V.Armstrong,J.Kidney,G.Chauhan,T.McManus,J.Gooskens1,V.vanderPloeg1,R.N.Sukhai2,A.C.T.M.Vossen1,C.McCaughey,H.J.O’Neill,D.Wyatt,P.V.Coyle.BelfastHealthandSocialR.Wolterbeek

3、3,E.C.J.Claas1,A.C.M.Kroes1.1DepartmentofMedicalCareTrust,Belfast,UnitedKingdomMicrobiology,LeidenUniversityMedicalCenter,Leiden,Netherlands,2DepartmentofPediatrics,LeidenUniversityMedicalCenter,Leiden,Purpose:Datafromapreviousstudysuggestedapossible

4、associationbetweenNetherlands,3DepartmentofBiostatistics,LeidenUniversityMedicalCenter,activeEBVinfectionandChronicObstructivePulmonaryDisease(COPD).Leiden,NetherlandsAcross-sectionalstudytoinvestigatethisobservationwasundertaken.Methods:Patientswith

5、earlyclinicalsymptomsofCOPDandcontrolsPurpose:Todescribetheepidemiologyandclinicalspectrumofrespiratory(‘healthysmokers’withnormalspirometry)wererecruited.Combinedvirusesdetectedinchildrenwithacuterespiratorytractinfection(ARTI).nasal/oropharyngealsw

6、absandinducedsputumswereobtainedfromeachMethods:Respiratoryvirusmultiplexreal-timePCRresultswereobtainedsubject.Totalnucleicacidswereextractedfromthesespecimens,andafromsinglespecimensofchildrenwithARTI(sampledwithin48hours),TaqManqPCRassaywasusedtod

7、etectandquantifyEBVDNA.Specimenpresentingtothehospitalduringthe2005–2006and2006–2007winterextractswerealsotestedforthepresenceof8humanherpesviruses(HSV-1,seasons.Amongchildrenaged0–36months,specificviralfindingswereHSV-2,VZV,CMV,EBV,HHV-6A,HHV-6B,HHV-7

8、)usingtheMobidiag®correlatedtopresentingsymptomsandclinicaloutcomebymultivariateProve-it’HerpesTubeArrayplatform.logisticregressionanalysis.Results:EBVDNAwasdetectedsignificantlymoreoftenamongsttheCOPDResults:Atotalof274childrenwithARTI(86.1%0−36month