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1、VeterinaryMicrobiology,5(1980)265--276265ElsevierScientificPublishingCompany,Amsterdam--PrintedinTheNetherlandsPROPERTIESOFACORONAVIRUSISOLATEDFROMACOWWITHEPIZOOTICDIARRHEAH.AKASHI,Y.INABA,Y.MIURA,S.TOKUHISA,K.SATOandK.SATODANationalInstituteofAnimalHealth,Tsukuba,Ibaraki(Japan)(Accept
2、ed21May1980)ABSTRACTAkashi,H.,Inaba,Y.,Miura,Y.,Tokuhisa,S.,Sato,K.andSatoda,K.,1980.Propertiesofacoronavirusisolatedfromacowwithepizooticdiarrhea.Vet.Microbiol.,5:265--276.m,Acoronavirus(Kakegawaisolate)isolatedfromacowwithepizooticdiarrheawasgrowninBEK-1cellsandexaminedforbiophysical
3、andbiochemicalproperties.TheKakegawaisolatewasabletoreplicateinthepresenceorabsenceof5-iodo-2'-deoxy-uridine,indicatingthatitsviralnucleicacidwasRNA.Itwashighlysensitivetoetherandchloroform,andmoderatelysensitivetotrypsinandheat.Itwas,however,readilystabilizedbytreatmentwithcationat50°
4、Cfor1h.ItsinfectivitywasslightlyreducedatpH3.0.Theviruspassedthroughamembranefilterof200nmporesize,butnotthroughoneof100nmporesize.Thebuoyantdensityoftheviruswasdeterminedinasucrosedensitygradient.Thepeakofinfectivityandhemagglutininactivitywasfoundatadensityof1.182.Neutralizationandhe
5、magglutinationinhibitiontestsshowedacloseserologicalrelationshipbetweentheKakegawaisolateandtheAmericanstrainofcalfdiarrheacoronavirus.INTRODUCTIONBovinecoronavirusescausingneonatalcalfdiarrheahavebeenisolatedintheU.S.A.(Stairetal.,1972;Doughrietal.,1976)andinEnglandandDenmark(Bridgere
6、tah,1978b).ThepresenceofthevirushasalsobeenreportedinBelgiumonthebasisofaserologicalsurvey(Zygraichetal.,1975)andbyimmunofluorescence(Wellemansetal.,1977a,b),andinCanadabyelectronmicroscopicandimmunofluorescenceobservations(Acresetal.,1975;Morinetal.,1976).Incontrasttotheconditionincal
7、ves,theetiologicalagentsofdiarrheainadultcattlehavebeenreportedinNewZealand(Homeretal.,1976;Durhametal.,1979),andrecentlyTakahashietal.(1980)isolatedacoronavirus-likeagentassociatedwithepizooticdiarrheainacowinJapan.ThisreportdealswiththephysicoChemicalcharacteristicsoftheJapaneseiso
