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1、_ArchivesArchVirol(1991)119:175-180Vfrology©Springer-Verlag1991PrintedinAustriaAcomparisonofthesensitivityandspecificityofsialodacryoadenitisvirus,Parker'sratcoronavirus,andmousehepatitisvirus-infectedcellsasasourceofantigenforthedetectionofantibodytor
2、atcoronavirusesD.H.Percy1,K.L.Williams1,andF.X.Paturzo21DepartmentofPathology,OntarioVeterinaryCollege,UniversityofGuelph,Guelph,Ontario,Canada2SectionofComparativeMedicine,YaleSchoolofMedicine,YaleUniversity,NewHaven,Connecticut,U.S.A.AcceptedJanuary7
3、,1991Summary.Sialodacryoadenitisvirus(SDAV)andParker'sratcoronavirus(PRC)aretworecognizedviralstrainswhichcausespontaneousdiseaseinthelaboratoryrat.Currentlythereisnorecognizedpracticalprocedurewhichwillaccuratelydifferentiateinfectionswiththesestrains
4、.UsingSDAV-andPRC-infectedL-2cellsasthesourceofantigen,andserafromratscollectedpostinoculationwitheitheroftheseviralstrains,theindirectfluorescentantibody(IFA)procedurewasusedtodeterminewhetherantibodytiterscouldbeusedtodifferentiateinfectionsfromtheho
5、mologousandheterologousvirus.Therewasnodetectabledifferenceinthesensitivityorspecificityofthesesystemsindetectingantibodytothehomologousorheterologousvirus.ThustherewasnoevidencethatSDAV-andPRC-infectedcellswouldservetodifferentiateantibodytothehomolog
6、ousvirususingtheIFAtechnique.Inaddition,an-tibodytitersweresimilarwhenmousehepatitisvirus(MHV)-infectedcellswereusedasthesourceofantigenfortheIFAtechnique.However,usingMHVorSDAV-infectedcellsasthesourceofantigen,therewasasignificantdifferenceinantibody
7、titerstothehomologousvirusdetectedusingtheimmunoenzymetechnique.IntroductionTheindirectfluorescenceantibody(IFA)testisarecognized,sensitivemethodtodetectantibodytospecificrodentviruses.Onemethodusedforthedetectionofcoronaviralantibodiesinrodentseracons
8、istsofequalproportionsofcellsinfectedwiththeJHMandSstrainsofmousehepatitisvirus(MHV)asasourceofantigen,togetherwithuninfectedcells.Usingthisasasourceofantigen,theindirectfluorescentantibodyprocedurehasproventobeatestsystemwith176D.H.Per
