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1、ArticleAppliedBiosafety,12(2)pp.100-108©ABSA2007EvaluationofSARS-CoronavirusDecontaminationProceduresAnne-MariePagat,RaphaelleSeux-Goepfert,CharlesLutsch,ValérieLecouturier,Jean-FrançoisSaluzzo,andIncaC.KustersSanofiPasteur,MarcyL’Etoile,FranceAbstract2006).Ithasbeenshownthat
2、theenvironmentisofex-tremeimportancetothestabilityofavirus.Forexample,WhenthefirstwaveoftheSARSepidemicseemedtoaviralmeaslesvaccinestraininsolutionisverysensitivetotemperatureandlight(deRizzoetal.,1990;Klammethavereacheditsconclusion,itwascompletelyunclearhowal.,1991;Sorodoce
3、tal.,1979),butveryresistantwhenthespreadoftheviruswouldevolve.Takingintoaccountallthestabilizer(solutionofsugarandaminoacids),usedintheuncertaintiesandanticipatingtheworst-casescenario,thefinalvaccineformulation,isadded(Melnick,1996).Amanylaboratoriesandvaccinemanufacturersst
4、artedworkingsimilarobservationwasmadeforinfluenzavirusincellonavaccineapproachagainstSARSinfection.Theresultsculturemediumsolution,whichwasverysensitivetodry-presentedheredescribetheevaluationofdecontaminationing,butmoreresistantwhenrecoveredfromallantoicpracticesperformedwit
5、hintheframeworkofaSARS-fluidofanembryonatedegg(Schafferetal.,1976).More-Coronavirus(SARS-CoV)vaccinedevelopmentproject.over,thesestudiesshowtherelativeresistanceofcertainWeshowthatittakes45daysatroomtemperaturetoviruses(poliovirus,hepatitisAvirus)foralkalinetreat-fullyinactiv
6、atethehumanSARS-CoV,whereasanenvelopedment(NaOH)(Salo&Cliver,1976)andthehighresis-virussuchastherabiesvirus,whentreatedinasimilarway,istanceofpoliovirusestodetergents(Kawanaetal.,1997;totallyinactivatedinthreedays.Moreover,theSARS-CoVisRutalaetal.,2000).Theprinciplelessonlear
7、nedfromtheseexperimentsveryresistanttoalkalinetreatmentand,evenmoresurpris-istheextremediversityofviralcharacteristicstodifferentingly,formaldehydefumigationisnotefficaciousonthedrieddecontaminatingconditions.Consequently,itisneces-virus(undertheconditionstested).Onlyheat(aut
8、oclave)andsarytovalidatethedecontaminationconditionsforeachhypochlor
