冠状病毒Coronavirusl论文-2004 The antiviral effect of interferon-beta against SARS-Coronavirus is not mediated by MxA protein.pdf

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1、JournalofClinicalVirology30(2004)211213Theantiviraleffectofinterferon-betaagainstSARS-CoronavirusisnotmediatedbyMxAproteinMartinSpiegela,AndreasPichlmaira,ElkeMühlbergerb,OttoHallera,FriedemannWebera,∗aAbteilungVirologie,InstitutfürMedizinischeMikrobiologieundHygiene,UniversitätFreiburg,D-79008F

2、reiburg,GermanybInstitutfürVirologiederPhilipps-UniversitätMarburg,35037Marburg,GermanyReceivedinrevisedform6November2003;accepted14November2003AbstractSevereacuterespiratorysyndrome(SARS)iscausedbyanovelcoronavirustermedSARS-CoV.Noantiviraltreatmenthasbeenestab-lishedsofar.Interferonsarecytokin

3、eswhichinducethesynthesisofseveralantivirallyactiveproteinsinthecell.Inthisstudy,wedemon-stratedthatmultiplicationofSARS-CoVincellculturecanbestronglyinhibitedbypretreatmentwithinterferon-beta.Interferon-alphaandinterferon-gamma,bycontrast,werelesseffective.ThehumanMxAproteinisoneofthemostpromin

4、entproteinsinducedbyinterferon-beta.Nevertheless,nointerferencewithSARS-CoVreplicationwasobservedinVerocellsstablyexpressingMxA.Therefore,otherinterferon-inducedproteinsmustberesponsibleforthestronginhibitoryeffectofinterferon-betaagainstSARS-CoV.©2003ElsevierB.V.Allrightsreserved.Keywords:SARS-

5、Coronavirus;Interferon;MxAprotein;Virusinhibition1.Introductioninestablishinganantiviralstate.TypeIIFN(IFN-
/)issynthesizedbymostcelltypesasadirectresponsetovirusSevereacuterespiratorysyndrome(SARS)isaninfec-infection,whereastypeIIIFN(IFN-)isproducedbyim-tiousdiseasewhichhasrecentlyemergedinCh

6、inaandmunecellsaftercontactwithantigen-presentingcells.Bothrapidlyspreadtoothercountries(Chanetal.,2003).TotypeIandtypeIIIFNsinducethesynthesisofdistinctdate,8098caseswith774deathsarereported(WHO,butpartiallyoverlappingsetsofmRNAswhichencodepro-2003).Intensiveresearchhasledtotheidentificationofte

7、inswithantiviral,antiproliferative,andimmunomodula-apositive-strandedRNAvirus,termedSARS-coronavirustoryproperties(deVeeretal.,2001).AmongthetypeI(SARS-CoV),astheetiologicagent(Drostenetal.,2003;IFN-inducedproteins,themainan