常用蛋白酶酶切位点.docx

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1、CleavageSitesTableforFusionProteins：PROTEASECOMPANYProteaseCaptureThrombinLeu-Val-Pro-Arg��Gly-SerAmersham-Biosciences,Novagen, SIGMA,RocheBenzamidine-AgaroseFactorXa Ile-Glu/Asp-Gly-Arg�� Amersham-Biosciences,NewEnglandBiolabs, RocheBenzamidine-AgaroseEnterokinaseAsp-Asp-Asp-Asp

2、-Lys��NewEnglandBiolabs, Novagen,RocheTrypsinInhibitor-AgaroseTEVproteaseGlu-Asn-Leu-Tyr-Phe-Gln��GlyInvitrogen–LifeTechnologies Ni-NTA(6Hisrecomb.TEV)PreScissionLeu-Glu-Val-Leu-Phe-Gln��Gly-ProAmersham-BiosciencesGSTrapforGSTfusionenzymeTAGZyme His-tagremovalbyExoproteolyticDige

3、stionQiagenNi-NTA(6Hisrecomb.enzyme)InteinSite dithiothreitolcleavageNewEnglandBiolabsDTTeliminationbydialysis HRV3CProtease  Leu-Glu-Val-Leu-Phe-Gln��Gly-ProNovagen   Ni-NTA(6Hisrecomb.enzyme)SUMOProteaserecognizethetertiarystructureoftheubiquitin-like(UBL)protein,SUMO Invitroge

4、n–LifeTechnologies,LifeSensorsNi-NTA(6Hisrecomb.enzyme)