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1、Inpharma1398-2Aug20031IFN-β-1b:potentantiviralactivityagainstSARScoronavirusInterferon(IFN)-α-2b,IFN-β-1bandIFN-γ-1binhibitreplicationoftheSARS*coronavirusinvitro,andIFN-β-1bisthemostpotent,reportresearchersfromGermany.TheyassessedtheantiviralpotentialofIFN-α-2b[‘IntronA’],IF
2、N-β-1b[‘Betaferon’]andIFN-γ-1b[‘Imukin’]againsttheFFM-1andHongKongisolatesoftheSARScoronavirus.Specifically,theresearchersvisuallyscoredthecytopathogeniceffectsoftheFFM-1andHongKongisolatesinVeroandCaco2cellsthatweretreatedwiththeIFNs24hoursbefore,andduring,infection.IFN-β-1b
3、wasthemostpotentantiviralinVerocells,followedbyIFN-γ-1bandthenIFN-α-2b[seetable].InCaco2cells,IFN-β-1bwasmorepotentthanIFN-α-2b,andIFN-γ-1bwascompletelyineffective.WhenthedrugswereaddedafterinfectionofVerocellswithFFM-1(1houradsorptionperiod),onlyIFN-β-1bwaseffective(EC50**=5
4、60IU/mL)."Therefore,onlyinterferonβcanbeusedasanantiviralagentafterinfection",commenttheresearchers.Finally,FFM-1virusreplicationinVerocellswasdose-dependentlyinhibitedbyIFN-β-1b(appliedbeforeandduringinfection).Table.InhibitionofcytopathogeniceffectsofFFM-1andHongKongisolate
5、sbyinterferonsECab50(IU/mL)SelectivityIndexVerocells/FFM-1isolateIFN-α-2b4950>2IFN-β-1b95>105IFN-γ-1b2500>4Verocells/HongKongisolateIFN-α-2b6500>1.5IFN-β-1b105>95IFN-γ-1b1700>5.9Caco2cells/FFM-1isolateIFN-α-2b1530>6.5IFN-β-1b21>476IFN-γ-1b>10000NCcCaco2cells/HongKongisolateIF
6、N-α-2b880>11.4IFN-β-1b9.2>1087IFN-γ-1b>10000NCaconcentrationrequiredtoinhibitthecytopathogeniceffectby50%bratiooftheconcentrationrequiredtoreducecellviabilityby50%totheconcentrationrequiredtoinhibitthecytopathogeniceffectby50%cnotcalculableTheresearchersconcludethat"interfero
7、nβcouldbethedrugofchoice,aloneorincombinationwithotherantiviraldrugs,inthetreatmentofSARS".*severeacuterespiratorysyndrome**concentrationrequiredtoinhibitthecytopathogeniceffectby50%CinatlJ,etal.TreatmentofSARSwithhumaninterferons.Lancet362:293-294,26Jul20038009432421173-8324
8、/10/1398-0001/$14.95Adis©2010SpringerInternationalPublishingAG.Allri
