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1、GModelCOLSUB-4158;No.ofPages4ARTICLEINPRESSColloidsandSurfacesB:Biointerfacesxxx(2010)xxx–xxxContentslistsavailableatScienceDirectColloidsandSurfacesB:Biointerfacesjournalhomepage:www.elsevier.com/locate/colsurfbPreparationof2-mercaptobenzothiazole-labeledimmuno-Auaggregate

2、sforSERS-basedimmunoassayChunyuanSong,ZhuyuanWang,JingYang,RuohuZhang,YipingCui∗AdvancedPhotonicsCenter,SoutheastUniversity,Nanjing210096,PRChinaarticleinfoabstractArticlehistory:Adetailedmethodforpreparationof2-mercaptobenzothiazole(MBT)-labeledimmuno-AuaggregatesasReceive

3、d24March2010anovelRamanprobeforSERS-basedimmunoassayhasbeenproposedinthispaper.Theformationkinet-Receivedinrevisedform9June2010icsofgoldaggregatesinducedbyMBTandtheimpactoftheamountoflabeledreporterontheaggregation,Accepted7July2010aswellasthefabricationofMBT-labeledimmuno-

4、AuaggregateswerecharacterizedcomprehensivelybyAvailableonlinexxxUV–visspectrophotometer.Meanwhile,achain-likemorphologyofaggregateswasmonitoredbyTEMimages.ExperimentalresultsshowthatthisRamantagcanactasanefficientlabelandanenhancedKeywords:RamansignalofSERS-basedimmunoassayc

5、anbeobtainedsuccessfullywiththisnovelprobe.ThisworkSurfaceenhancedRamanscattering(SERS)showsthemethodforthepreparationofimmuneprobeforSERS-basedimmunoassayusingMBTasAggregate2-Mercaptobenzothiazole(MBT)reporterandprovidesareferenceforthepreparationofimmuneprobewithotherRama

6、nmarkersforImmunoassaymulti-channelSERS-basedimmunoassay.Reporter©2010PublishedbyElsevierB.V.1.IntroductionclesresultsinaSERSsignalincreaseoftheRamanreporterandtheproteincouldbedeterminedbymeasuringtheRamansignalSurfaceenhancedRamanscattering(SERS),ananalyticalofthereporter

7、.However,theotheroneisperformedonasolidtechniquewhichprovidesthestructuralinformationofRamansubstrate[7–13],whichisapotentstrategyasanimmunochipforspectroscopytogetherwithultrasensitivedetectionlimits,opensmulti-channeldetection[14–16].Differentfromtheformer,thisupexcitingo

8、pportunitiesinthefieldofbiochemicalsensing[1–3].immunoassayisoftenperformedwithasan