资源描述：
《应用植入后大鼠体外全胚胎培养研究酒精对胚胎发育的影响.doc》由会员上传分享，免费在线阅读，更多相关内容在工程资料-天天文库。
1、应用植入后大鼠体外全胚胎培养研究酒精对胚胎发育的影响T生研究2000年第1期第29卷论著作者：屈T东张木忠吴德生吴玮王云波张雪刘发才单位：屈卫东（华西医科大学公共卫生学院，成都610041）；张本忠（兰州医学院卫生系毒理教研室，兰州730000）；吴徳生（华西医科大学公共卫生学院，成都610041）；吴玮（华西医科大学公共卫生学院，成都610041）；王云波（北京市东城区卫生防疫站，北京100009）；张雪（华西医科大学公共卫生学院，成都610041）；刘发才（华西医科大学公共卫生学院，成都610041）关键词：植入后全胚胎培养；酒精；胚胎发育摘要：为探讨孕期饮
2、酒对胚胎生长发育的影响，应用植入后全胚胎培养（wholeembryoculture,WEC）进行研究。将孕9.5天龄鼠胚胎移植于酒精终浓度分别为0、0.40、1.00、2.00.4.00g/L的全胚胎培养基中，于体外培养48小时，观察酒精对胚胎发育和器官形态分化影响。结果表明酒精对胚胎发育具有明显影响且呈剂量-效应关系，0.40g/L组胚胎生长发育指标和器官形态分化指标与对照组差异无显著性(P>0.05),1.00g/L组显著影响中脑、前脑、神经管、卵黄囊循环和翻转等指标,2.00g/L以上组各指标显著低于对照组(P<0.05),胚胎致畸率和死亡率显著增高。结论
3、认为酒精具有发育毒性和致畸性，酒精作用的主要靶器官是脑，酒精对卵黄囊发育分化和DNA合成的影响可能与其发育异常有关。屮图分类号：Q132.7R595.6文献标识码：A文章编号：1000-8020(2000)01-0017-03Studyontheeffectofalcoholonembryonicdevelopmentbyusinginvitropost-implantationratwholeembryocultureQuWeidong,ZhangBenzhong,WuDesheng,WuWei,etal.(DepartmentofEnvironmentaiH
4、ealth,SchoolofPublicHealth,Chengdu610041,China)Abstract：Inordertoexploretheeffectsofdrinkingalcoholduringpregnancyonembryonicdevelopmentanditsmechanism,apost-implantationwho1eembryoculture(WEC)techniquewasused・The9.5dayratembryoswereexplantedinratserummedium(immediate1ycentrifugalser
5、um,ICS)withalcohol(0,0.4,1.0,2.00and4.00^/L),andculturedfor48hours・Theindexofembryodevelopmentandmorphologicalscoresinducedbyalcoholwereobserved・Theresultshowedthatalcoholhadobviouslyeffectsonthedevelopmentandgrowthofembryoswithadose-responserelationship・Embryonicdevelopmentof0.4g/Lg
6、roupwasnotsignificantlydifferentfromthecontrolgroup,whereas1・Og/Lgroupcouldinterferewiththedevelopmentscoreofmid-brain,forebrain,neurotube,andvisceralyolksac(VYS)circleobviously.Allscoresofthe2.00g/Lgroupweresignificantlylowerthanthatofcontrolgroup(PV0.05)・Moreover,therateofembryolet
7、halityandteratogenecitywereobviousincreased・Itisconcludedthatalcoholhasdevelopmentaltoxicityandteratogenicity.Thetargetorganaffectedbyalcoholisbrain.TheeffectsofalcoholonthedevelopmentaldifferentiationofvisceralyolksacandDNAsynthesisareprobablyrelatedtoitsdevelopmentalabnormalities・K
8、eywords:post
