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1、中国农业科学2008,41(8):2279-2285ScientiaAgriculturaSinicadoi:10.3864/j.issn.0578-1752.2008.08.0092+Ca信号参与铝诱导黑麦根系分泌有机酸的调控11112唐新莲，黎晓峰，凌桂芝，顾明华，玉永雄12（广西大学农学院，南宁530005；西南大学动物科技学院，重庆400716）摘要：【目的】揭示铝诱导根系分泌有机酸的机制，探讨胞质钙信号对铝诱导黑麦根系分泌有机酸的调控作2+用。【方法】采用药理学研究方法和激光共聚焦扫描显微技术探讨铝胁迫
2、下根尖胞质游离钙离子浓度（[Ca]cyt）2+及其与有机酸分泌的关系。【结果】铝不仅诱导黑麦根系分泌柠檬酸和苹果酸，而且使根尖细胞Ca的荧光强度增2+2+2+强、波动加剧。铝引起的根尖细胞Ca荧光强度的变化在受Ca通道抑制剂异搏定、胞内Ca通道阻断剂辽红干2+扰后，显著减少了铝诱导的有机酸分泌。铝诱导的根尖[Ca]cyt的升高及有机酸分泌还受CaM阻断剂三氟拉嗪的干2+2+扰和抑制。钙螯合剂EGTA处理不仅减弱了铝诱导的Ca荧光信号、加大了Ca信号的波动幅度，而且显著抑制铝2+诱导的柠檬酸分泌。此外，在铝胁迫下，
3、根系有机酸分泌受阴离子通道抑制剂尼氟灭酸的抑制。【结论】根尖[Ca]cyt可能是介导铝诱导黑麦根系分泌有机酸的胞内信号因子，而质膜上的阴离子通道可能是铝诱导的钙信号传导途径中的下游效应器。2+关键词：铝；黑麦；有机酸分泌；胞质Ca；信号转导2+TheInvolvementofCaSignalintheRegulationofAl-InducedSecretionofOrganicAcidsinRye11112TANGXin-lian,LIXiao-feng,LINGGui-zhi,GUMing-hua,YUYon
4、g-xiong12(CollegeofAgriculture,GuangxiUniversity,Nanning530005;CollegeofAnimalScienceandTechnology,South-westUniversity,Chongqing400716)2+Abstract:【Objective】TheinvolvementofintracellularCainregulationofAl-inducedsecretionoforganicacidsfromrootsofrye(Secalece
5、realeL.)wasinvestigatedinpresentstudytoelucidatethemechanismsforAlresistanceinplants.【Method】In2+presentstudy,[Ca]cytanditsinvolvementinAl-inducedsecretionoforganicacidsfromrootswerestudiedbypharmacologicalmethodandconfocalscaningmicroscopytechnology.【Rresult
6、】Alnotonlyinducedthesecretionofbothcitrateandmalate,butalso2+2+elevatedthelevelsof[Ca]cytofrootapicesandenhancedthefluctuationof[Ca]cyt.However,theinhibitionofAl-induced2+secretionoforganicacidswasfollowedbytheinterruptionofAl-inducedalternationin[Ca]cytfulor
7、escenceintensityofroots2+treatedwithVerapamilorrutheniumred.Moreover,[Ca]cytandthesecretionoforganicacidswerealsointerferedorinhibitedbythetreatmentwithTrifluoperazine(CaMantagonist).Furthermore,Al-inducedsecretionofcitratewasblockedbythetreatmentwith2+2+EGTA
8、,whichresultedinawidefluctuationof[Ca]cytandaweakCafluorescenceintensity.Ontheotherway,thesecretionoforganicacidswasinhibitedbythetreatmentwithanionchannelinhibitor,niflumicacid.【Conclusi
