CARTHAMUS YELLOW-羟基红花黄色素.pdf

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1、CARTHAMUSYELLOWPreparedatthe51stJECFA(1998),publishedinFNP52Add6(1998)supersedingspecificationspreparedatthe49thJECFA(1998),publishedinFNP52Add5(1997).Metalsandarsenicspecificationsrevisedatthe59thJECFA(2002).NoADIallocatedatthe29thJECFAin1985.SYNONYMSSaffl

2、oweryellow,CINaturalYellow5DEFINITIONCarthamusYellow,aflavonoid,isobtainedbyextractingthecorolla(petals)ofCarthamustinctoriusL.withwaterorslightlyacidifiedwateranddryingtheextract.TheprincipalcolouringmattersaresafflominA(hydroxysaffloryellowA)andsafflomine

3、B(saffloryellowB).Besidesthecolourpigmentscarthamusyellowconsistsofsugars,saltsand/orproteinsnaturallyoccurringinthesourcematerials.Foodgradematerialssuchasdextrinmaybeaddedascarriersformanufacturingdry,powdereditemsofcommerce.C.A.SnumberI.78281-02-4(Safflo

4、minA)II.120478-62-8(SafflominB)ChemicalformulaI.C27H32O16(SafflominA)II.C48H54O27(SafflominB)StructuralformulaSafflominASafflominBFormulaweightI.612.5(SafflominA)II.1062(SafflominB)AssayContentofcolouringmatters(calculatedassafflominA)notlessthandeclaredDES

5、CRIPTIONYellowtodarkbrowncrystals,paste,powderorliquidwithafaintcharacteristicodour.FUNCTIONALUSESColourCHARACTERISTICSIDENTIFICATIONSolubility(Vol.4)Verysolubleinwater,practicallyinsolubleinetherandethanolSpectrophotometryAsolutionofthesampleincitricacid/d

6、isodiumhydrogenphosphatebuffer(Vol.4)solution(pH5.0)isyellowandshowsanabsorptionmaximumat400-408nm.ThinlayerActivatecellulosefor20minat60-80°andprepareaTLCplate.Prepareachromatography10%solutionofthesampleinmethanolandapply20µltotheplate.Allowto(Vol.4)dryan

7、ddevelopusingamixtureofn-butanol,aceticacidandwater(4:1:2byvolume)untilthesolventfronthasascendedabout10cm.Allowtodry.ThemaincomponentsofcarthamusyellowappearastwoyellowspotswithRfvaluesintherange0.2-0.5.ColourreactionMakethesolutionofthesampleinwateralkali

8、neby10%sodiumhydroxidesolution;thecolourchangesfromyellowtoorange-yellowPURITYSyntheticdyesBasicdyes:To1gofthesampleadd100mlof1%sodiumhydroxidesolution,andmixwell.Extract30mlofthissolutionwith15mlofeth