srebp-1c基因多态性与非酒精性脂肪性肝病的关系研究

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1、西南国防医药2014年2月第24卷第2期·论著·SREBP一1c基因多态性与非酒精性脂肪性肝病的关系研究张海英，高燕翔，冯晓凡，张乾勇，糜漫天[摘要]目的研究固醇调节元件结合蛋白一lc(SREBP—lc)基因多态性与非酒精性脂肪性肝病(NAFLD)的关系。方法选取152例NAFLD患者和146例未患脂肪肝人员，收集血液样本检测血脂水平，采用聚合酶链反应一限制线片段长度多态性(PCR—RFLP)技术分析SREBP一1c基因18号外显子54G／C多态性。结果NAFLD病例组TC、TG和LDL．C水平均明显高于对照组(P<0．05)。两组的SREBP一1c18号外显子5

2、4G／C单核苷酸多态性均以GG型的比例最高，两组基因型频率分布有统计学差异(X=6．1096，P<0．05)，NAFLD病例组中C等位基因频率明显高于对照组(=6．2520，P<0．05)。C等位基因携带者患NAFLD的风险是G等位基因的1．6484倍(OR=1．6484，95％CI：1．3233—10．0984)。结论研究对象的血脂水平异常与NAFLD的患病有显著相关性，参与糖代谢、脂代谢的SREBP-lc基因l8号外显子54G／C呈现多态性，其C等位基因可能会使个体的NAFLD发病风险增加。[关键词]固醇调节元件结合蛋白．1c；非酒精性脂肪性肝病；基因多态性；

3、等位基因中图分类号R575．29文献标识码A文章编号1004-0188(2014)02-0117-04doi：10．3969／j．issn．1004-0188．2014．02．001Researchoncorrelationbetweengenepolymorphismofsterolregulatoryelementbindingproteinicandnon·alcoholicfattyliverdiseaseZhangHaiying，GaoYanxiang，FengXiaofan，ZhangQianyong，MiMantian1．ResearchCenter

4、forNutritionandFoodSafety．theThirdMilitaryMedicalUniversity／ChongqingKeyLaboratoryofNutritionandFoodSatety，Chongqing400038，China；2．ChengduOmmayHeahhExaminationHospital，Chengdu，Sichuan，610041，China[Abstract]ObjectiveTostudythecorrelationbetweengenepolymorphismofsterolregulatoryelementb

5、indingproteinlc(SREBP一1c)andnon-alcoholicfattyliverdisease(NAFLD)．Methods152patientswithNAFLDand146personswithoutfattyliverwereselectedasthesubjects．Theirbloodsampleswerecollected，andthebloodfatlevelsweredetected．Polymerasechainreaction—restrictionfragmentlengthpolymorphism(PCR—RFLP)w

6、asusedtoanalyzethepolymorphismofNO．18exon54G／CofSREBP一1cgene．ResultsThelevelsofTC，TG，andLDL—CintheNAFLDgroupweresignificantlyhigherthanthoseinthecontrolgroup(P<0．05)．TheproportionofGGtypewasthehighestinthemononucleotidepolymorphismofSREBP·1cNO．18exon54G／Cinbothgroups．Thereweresignific

7、antdifferencesinthegenotypicfrequencybetweenthetwogroups(=6．1096，P<0．05)．ThefrequencyofCallelegenotypeinNAFLDgroupwassignificantlyhigherthanthatinthecontrolgroup(X2=6．2520，P<0．05)．TheonsetriskdegreeofNAFLDinthecarriersofCallelewas1．6484timesofthatinthecarriersofGallele(OR=1．6484，95％C／

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