PIWILl蛋白与DICER酶在肝细胞癌中的表达及意义-论文.pdf

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1、·9O8·中华肝胆外科杂志2013年12月第19卷第12期ChinJHepatobiliarySurg，December2013，Vo1．19，No．12·论著·PIWIL1蛋白与DICER酶在肝细胞癌中的表达及意义范正军孙涛杨飞龙程波彭飞周闯【摘要】目的探讨P1wII1蛋白与DICER酶在肝细胞癌组织中的表达及临床意义。方法免疫组化检测47例肝细胞癌标本中PIWIL1与DICER的表达。蛋白印迹法检测31例肝细胞癌组织中PIWIL1与DICER的表达。分析PIWII1与DICER表达及与临床特征的关系。结果PIWlI1在癌组织中高表达，在正常肝组织中低表达

2、(P

3、carcinomaFANZheng—un，SUNTa0，yANGFei—long，CHENGBo，PENGFei，ZH0UChuang．eFirstAffiliatedHospital0fZhengzhouUniversity，Zhengzhou450052，ChinaC0rre声。ngauthor：FANZheng—Un，Email：droider@163．com[Abstract]ObjectiveToexploretheexpressionofPIWIL1proteinandDICERenzymeinhepatocellularcarcinoma(HC

4、C)andtheirsignificance．MethodsImmunohistochemica1methodwasusedtodetecttheexpressionofPIWIL1andDICERin47casesofHCCandtheadjacentHCCtissues．WesternblotmethodwasusedtOdetecttheexpressionofPIWIL1andDICERin31casesoffreshHCCtissuesandtheiradjacentHCCtissues．TherelationshipbetweenPIWIL1an

5、dDICERandtheirrelationshipswereanalysedwithclinica1features．12casesofnorma1livertissueswereusedascontrolgroup．ResultsTheexpressionofPIWIL1washighinHCCbutlOWinnormal1ivertissues(P

6、velycorrelatedwithinvasiontoadjacenttissuesandhistologicaldiffer—entiation(Pd0．O5)．TheexpressionofDICERwasnegativelycorrelatedwithinvasiontotheadacenttissuesandhistologica1differentiation(Pd0．05)．ThereW38anegativecorrelationbetweenPIWIIlandDICER(P<0．05)．ConclusionsHighexpressionofP

7、IWII1andlow／missingexpressionofDICERwasrelatedtopathologicaldifferentiationandinvasionofadjacenttissues．[Keywords]Hepatocel1ularcarcinoma；Piwilikeprotein；Ribonuclease1I，DICER肝细胞癌(hepatoce11ularcarcinoma，HCC)具原(Piwi-likeprotein1，PIWILl／H1wI)为新发现有发病率高、难以早期发现、病死率高等特点。我国的恶性肿瘤基因蛋白。PIWII

8、1与多种肿瘤的发生每年因肝细胞癌死亡的人数在恶性肿瘤