两面针提取物抗肝癌谱-效关系研究-论文.pdf

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1、·1102·中草焉ChineseTraditionalandHerbalDrugs第45卷第8期2014年4月●药理与临床●两面针提取物抗肝癌谱．效关系研究王宏虹一，刘华钢，黄慧学，刘而敏，中庆荣，曹利娟i．柳州市妇幼保健院，广西柳州5450012．广西医科大学药学院，广西南宁5300213．广西壮族自治区人民医院，广西南宁5300214．柳州市人民医院，广西柳州545001摘要：目的研究两面针药材提取物HPLC指纹图谱与抗肝癌活性之间的关系。方法以人肝癌细胞株HepG2为药效模型，采用MTT法测定33批不同

2、产地两面针药材提取物的抗肝癌活性，采用逐步后退法将量化特征峰面积与MTT法测得两面针提取物抗肿瘤活性的结果结合，构建两面针药材提取物抗肝癌的谱效关系方程。结果建立的两面针药材提取物HPLC指纹图谱相似度达到0．7以上，经相似度软件筛选出24个特征指纹峰，用逐步后退法拟合两面针药材提取物抗肝癌谱效关系方程，该方程具有统计学意义和实际意义，可较准确地预测两面针药材提取物的抗肝癌活性。结论两面针药材提取物抗肝癌活性是多种成分协同、互补、制约相互作用的结果，根据方程可预测肿瘤细胞的敏感性。关键词：两面针药材；HepG

3、2细胞；谱效关系；抗肝癌；指纹图谱中图分类号：R285．5文献标志码：A文章编号：0253．2670(2014)08—1102—08DOI：10．7501~．issn．0253—2670．2014．08．012Antihepatomaspectrum—effectrelationshipofextractfromZanthoxyliRadixWANGHong．hong，LIUHua—gang，HUANGHui．xue，LIULi—min2，SHENQing—rong，CAOLi~uan1．LiuzhouMat

4、ernalandChildrenHealthHospital，Liuzhou545001，China2．SchoolofPharmaceuticalSciences，GuangxiMedicalUniversity,Nanning530021，China3．ThePeople’SHospitalofGuangxiZhuangzuAutonomousRegion，Nanning530021，China4．ThePeople’SHospitalofLiuzhou，Liuzhou545001，ChinaAbstra

5、ct：ObjectiveTostudytherelationshipbetweenHPLCfingerprintandantihepatomaactivityoftheextractfromZanthoxyliRad~．MethodsZanthoxyliRad&fingerprintwasestablishedandhumanhepaticcarcinomacelllineHepG2waschosenaseficacymode1．TheantihepatomaactivityoftheextractsinZa

6、nthoxyliRadixof33batchsfromdiferenthabitatswasdeterminedbyMTI"method．A_~erintegratedtheo3eaofcharacteristicpeakwiththeantihepatomaactivityofZan~oxyliRadix,thehepaticc~cinoma“spectrum-efect”relationshipWasestablishedbystep-backtechnique．ResultsTheHPLC—finger

7、printofZan~oxyliRadixwasestablished，andthesimilarityoffingerprinthadreachedabove0．7．Alldataweredonewiththechromatographicfingerprintsimilarityevaluationsystemsoftware，chosenout24fingerprintpeaks．Thespectrum-efectrelationshiponHepG2Wasestablishedandthisrelat

8、ionshipWassignificantlydiferent．TheresultsindicatedthatthemultiplelinearregressioncouldpredicttheantihepatomaactivitytoHepG2withstatisticalandpracticalsignificance．ConclusionThespectrum-efectrelationsh