miR-224在原发性肝癌患者血清中的表达及意义-论文.pdf

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1、现代生物医学进展www~shengwuyixue．comProgressinModernBiome~cmeVoL14NO．4FEB．2014·621·DOI：10．13241／j．cnki．pmb．2014．04．005miR．224在原发性肝癌患者血清中的表达及意义郭晓东余灵祥杨美郭超楠李志伟△(解放军第302医院北京100039)摘要目的：原发性肝癌(primaryhepatocellularcarcinoma，PHC)作为常见的恶性程度极高的肿瘤，严重威胁着人类的生命。miR-224是近年来发现的一个肿瘤相关mi

2、RNA分子，在肿瘤的发生及发展过程中发挥着重要的作用。本研究通过测定原发性肝癌患者血清中miR-224的表达水平，探讨血清miR一224与原发性肝癌预后的关系。方法：采用实时荧光定量RT-PCR(Real-timeRT．PCR)方法，分别检测40例原发性肝癌患者，20例慢性肝炎患者，2O例慢性肝硬化患者及20例正常人的血清标本中miR-224的表达水平。分析血清miR-224的表达水平与AFP和MMP．9的相关性。结果：原发性肝癌患者血清miR-224的表达水平明显高于正常人、慢性肝炎和慢性肝硬化患者(P

3、皮尔森相关分析结果显示原发性肝癌患者血清miR-224的表达与AFP和MMP．9呈正相关。血清miR-224低表达组术后复发／转移率显著低于高表达组，术后生存率则高于高表达组(P<0．01)。结论：miR-224在原发性肝癌患者的血清中呈高表达，其血清表达水平与原发性肝癌的临床预后密切相关。这提示我们，miR-224可能成为新的原发性肝癌检测标记物和潜在的原发性肝癌预后分子标志物。关键词：原发性肝癌；血清；miR一224；预后中图分类号：R735．7文献标识码：A文章编号：1673—6273(2014)04—621—0

4、3ExpressionandSignificanceofmiR．224inSerumonPrimaryHepatocellularCarcinoma*GUOXiao—dong,YULing-xiang,YANGMei．GUOChao-nan,LIZhi-wei△f302HospitalofPLA,Beijing,100039,China)ABSTRACTObjective：Asacommonmalignanttumor，theprimarylivercancer(primaryhepatocellularcarcino

5、ma，PHC)hasseriouslythreatenedthehumanlife．Recently，miR一224hasbeendiscoveredastherelativemicromasmoleculesoftumorthatplayedallimportantroleontheoccurrenceanddevelopmentprocessoftumor．ThisresearchaimstoinvestigatetheexpressionofmiR-224inserumonprimaryhepatocellula

6、rcarcinoma，aswellastherelationshipwiththeprognosisofprimaryhepatocellularcarcinoma．Methods：Thebloodsampleswerecollectedfrom40patientswithPHC，20patientswithchronichepatitis，20patientswithchronichepaticcirrhosisand20healthypeople．TheexpressionlevelsofmiR-224inthes

7、erull'1weremeasuredbyreversetranscriptionpolymerasechainreaction．ThecorelationsofmiRNA一224expression，traumaticinjuryandtheprognosisofprimaryhepatocellularcarcinomawereanalyzed．Results：TheexpressionlevelofmiR-224inpatientswithPHCwassignificantlyhigherthanthoseofp

8、atientswithbenigndiseasesandhealthypeoplefP<0．05)．Pearson’ScorelationanalysisshowedthattheexpressionofmiR-224inserumhadaclosecorelationwithAFPandMMP-9rP<0．05)．Therecu