骨髓间充质干细胞移植对颅脑损伤大鼠神经功能的影响-论文.pdf

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1、40卷第9Mav2Ol4，V0140，No．9论著●骨髓间充质干细胞移植对颅脑损伤大鼠神经功能的影响万兴王文宏樊欣鑫【摘要】目的研究骨髓间充质干细胞(BMScs)移植对颅脑损伤大鼠神经功能的影响。方法分离、纯化大鼠BMSCs，并用左旋多聚赖氨酸一超顺磁性纳米铁颗粒(PII：SPIO)复合物对BMSCs进行标记。采用改良Feeney法制成创伤性颅脑损伤大鼠模型6O只，随机均分为两组。实验组在脑损伤部位的对侧立体定向移植PLI，SPIO标记的BMSCs；对照组注入等量的生理盐水。在移植后第1、3、7、14和28天时采用改良神经功能损伤严重程度评分(mNSS)

2、对各组大鼠进行评分；然后行MRI扫描，观察脑实质内的信号变化及BMSCs在脑内的迁移情况。应用组织学检查，观察脑损伤部位的病理变化。结果含量为25／xgFe／ml的PILSPIO培养液能成功标记BMSCs(标记率为100)。实验组大鼠在细胞移植后的第3、7、14天时mNSS低于对照组(P

3、】A【文章编号102533685(2014)09—1006—05Effectsofbonemesenchymalnlal'rowstemcellsOilneurologicalfunctionofratswithtraumaticbraininjuryWAN，WANGWenhong，FANXinxin．DepartmentofNeurosurgery，AffiliatedHospital，SoutheastUniversity，Nanjing210009，CHINA[Abstract]0bjectiveToexploretheeffectsofbonem

4、esenchymalmarrowsterncells(BMSCs)onneurologicalfunctionofratswithtraumaticbraininiury(TBI)．MethodsTheBMSCswereisolatedandpurifiedfromratbonemarrow，whichwerethenlabeledwithacomplexofpoly-lysine(PII)andSPI()．TheTBImode1wasestablishedbyfree-fallinginstrumentofFeeneyin60maleSDrats．wh

5、ichthenwererandomlydiviededintotwogroups．ThePLL-SPIO-labeledBMSCswerestereotactical1vtransplantedintothebrainoppositetotheTBIsideingroup八ThesamevolumeofnormalsalineinsteadofBMSCswasinjectedingroupBasthecontro1．TheevaluationwithmodifiedNeurologica1SeverityScore(mNSS)wasperformedon

6、the1st，3rd，7th，14thand28thdayafterTBI．ThesignalchangesinthebrainandthedistributionandmigrationofSPI(labeledBMSCswereobservedbyMRI．PathologicalchangeswereobservedintheiNuriedpartofthebrainbyHEdyeingandPrussianbluestaining．ResultsThePLL-SPIO25ggFe／mlculturesolutionsuccessfullylabel

7、edBMSCswithasuccessrateof100．ThemNSSscoresweresignificantlyloweringroupAthanthoseingroupBonthe3rd，7thand14thdayafterTBI(P

8、otactictransplantationofBMSCscouldimprov