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1、Chapter2ElectrophoresisTechniques08August2021GeneticEngineering2TheintroductionofelectrophoresistechniquesTheclassificationofelectrophoresistechniquesTheprinciplesandoperationsofelectrophoresistechniquesTheapplicationofelectrophoresistechniquesSummari

2、ze(Discussionintheclass)08August2021GeneticEngineering3电泳技术简介电泳技术分类常见电泳技术的原理及操作方法电泳技术的应用本章主要内容08August2021GeneticEngineering41.1TheintroductionofelectrophoresistechniquesElectrophoresisisatechniqueusedtoseparateandsometimespurifymacromolecules大分子-espe

3、ciallyproteinsandnucleicacids-thatdifferinsize,chargeorconformation构象.Incontrasttoproteins,whichcanhaveeitherapositiveornegativecharge,nucleicacidshaveaconsistent一定的negativechargeimparted给予bytheirphosphate磷酸的backbone,andmigrate迁移towardtheanode阳极.08Aug

4、ust2021GeneticEngineering51.1TheintroductionofelectrophoresistechniquesProteinsandnucleicacidsareelectrophoresedwithinamatrixor"gel".Mostcommonly,thegeliscastintheshapeofathinslab,withwellsforloadingthesample.Thegelisimmersedwithinanelectrophoresisbuf

5、ferthatprovidesionstocarryacurrentandsometypeofbuffertomaintainthepHatarelativelyconstantvalue.蛋白质或核酸能够在介质当中进行移动.最常见的是利用模具制备具有上样孔的凝胶板，然后将凝胶进入到含有电解质或能够维持PH值处于相对稳定的电泳缓冲液当中。08August2021GeneticEngineering61.2TheclassificationofelectrophoresistechniquesAcc

6、ordingtothedifferenceofthemediumCelluloseacetatemembraneelectrophoresisAgarosegelelectrophoresisSDSPolyacrylamidegelelectrophoresisCapillaryelectrophoresisAccordingtothedifferenceofanalyzingsubjectsNucleicacidelectrophoresisProteinorpeptideselectropho

7、resis08August2021GeneticEngineering71.1电泳技术分类依据电泳介质分类醋酸纤维素膜电泳琼脂糖凝胶电泳SDS-聚丙烯酰胺凝胶电泳毛细管电泳依据不同的分析对象核酸电泳蛋白或多肽电泳08August2021GeneticEngineering81.2.1Celluloseacetatemembraneelectrophoresis醋酸纤维素膜Theprinciplesofcelluloseacetatemembraneelectrophoresis+H3N-P-COO

8、H-H++H++H3N-P-COO--H++H+H2N-P-COO-PositiveionZwitterionNegativeionTheproteinwhichcanhaveeitherapositiveornegativechargeinthesolutionatdifferentpH.两性离子阳离子阴离子08August2021GeneticEngineering91.2.1Celluloseacetatemembraneelectrophoresis醋酸纤维素膜Themed