结核分枝杆菌研究论文

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1、结核分枝杆菌研究论文第8页共8页结核分枝杆菌研究论文第8页共8页结核分枝杆菌研究论文第8页共8页结核分枝杆菌研究论文第8页共8页结核分枝杆菌研究论文第8页共8页结核分枝杆菌研究论文第8页共8页结核分枝杆菌研究论文第8页共8页【关键词】结核分枝杆菌ImmuneresponsesandprotectiveefficacyinducedinmicebyMycobacteriumtuberculosisMPT64ESAT6fusionprotein【Abstract】AIM:Toevaluatehumoralandcellmediatedimmunerespons

2、esbyMPT64andESAT6fusionproteinsandtotestprotectiveefficacyagainstMycobacteriumtuberculosis(MTB)challenge.METHODS:BALB/cmicewereimmunized3timesat2weekintervalsubcutaneouslyonthebackwithfusionproteinMPT64ESAT6.Inthespleenlymphocytesofimmunizedmicestimulationindex(SI)wasmeasuredbyMTT

3、methodandthelevelofsecretedIFNγwasdetectedbyELISA.SomeofvaccinatedBALB/cmicebyfusionproteinswereintravenouslyinfectedwith1×105CFUMTBstrainH37Rv.Fourweekslater，bacterialloadinspleenswasdetermined.RESULTS:ThetiterofseraspecificantibodyinBALB/cmiceimmunizedwithfusionexpressionprotein

4、MPT64ESAT6was1∶1500.TheSIoflymphocytesinfusionproteinimmunizedgroupwas2.23,significantlyhigherthanthatofsalineimmunizedgroup(0.88).TheIFNγlevelinculturesupernatantofspleenlymphocytesfromthemiceimmunizedwithfusionproteinswere(4.28±0.27)μg/L,significantlyhigherthanthatofsalineimmuni

5、zedgroup［(0.48±0.17)μg/L,P<0.05］,butlowerthanthatofBacillusCalmetteGuerin(BCG)immunizedgroup［(5.18±0.31)μg/L］.Comparedwithsalineimmunizedmice（bacterialloadwas6.45±0.17）,dramaticreductionswereobservedforMTBreplicationinthespleenfromBALB/cmiceimmunizedwithfusionproteins(bacteriaload

6、was5.04±0.11)followingasubsequentchallenge,buttheprotectiveefficacyinmiceimmunizedwithMPT64ESAT6wasnotasgoodasthatofBCGimmunizedgroup(bacterialloadwas4.38±0.22).CONCLUSION:MPT64andESAT6fusionproteinscouldbeusedasanovelcomponentofthenewTBvaccine.【Keywords】Mycobacteriumtuberculosis；

7、vaccine；MPT64；ESAT6；fusionexpression【摘要】目的：测定MPT64与ESAT6融合蛋白在小鼠体内诱导的免疫应答及保护力.方法：将MPT64与ESAT6的融合蛋白皮下免疫小鼠3次，每次间隔2wk，ELISA测定免疫小鼠血清特异性抗体的滴度.最后一次免疫完成后4wk，分离部分免疫小鼠脾淋巴细胞，MTT法检测淋巴细胞刺激指数，ELISA检测悬液中IFNγ水平.另一部分免疫的BALB/c小鼠经尾静脉感染MTB毒株H37Rv，4wk后计数脾脏细菌负荷数.结果：MPT64ESAT6融合蛋白免疫小鼠血清特异性抗体滴度1∶1500，免疫小

8、鼠后淋巴细胞刺激增殖指数为2.23，而生理盐水免疫组只有0.88；