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1、RESEARCHPAPERNewBiotechnologyVolume28,Number2February2011AnovelorganicsolventtolerantproteasefromanewlyisolatedGeomicrobiumsp.EMB2ResearchPaper(MTCC10310):productionoptimizationbyresponsesurfacemethodology1231,RamKaran,S.P.Singh,SanjayKapoorandS.K.Khare1DepartmentofChemistry,IndianInstituteofTec
2、hnology,Delhi,NewDelhi,India2DepartmentofBiosciences,SaurashtraUniversity,Rajkot,Gujarat,India3DepartmentofPlantMolecularBiology,UniversityofDelhiSouthCampus,NewDelhi,IndiaThirty-eighthaloalkaliphilicbacterialstrainswereisolatedfromSambharSaltLake,Indiaandscreenedfortheirabilitytosecretehaloalkali
3、philicproteases.Amongthem,amoderatelyhalophilic,mesophilicandalkaliphilicpotentstrainGeomicrobiumsp.EMB2producedanextracellularprotease,whichwasremarkablystableinorganicsolvents,salt,surfactants,detergentsandalkalinepH.Statisticallybasedexperimentaldesignswereappliedtostudytheinteractionsandoptimi
4、zationofmediumconstituentsforefficientproteaseproductionbyGeomicrobiumsp.EMB2.Anoverall20-foldincreaseinproteaseproductionwasachievedintheoptimizedmedium(721U/ml)ascomparedwiththeunoptimizedmedium(37U/ml).Thehighproductionlevelcoupledwithnovelpropertiesmakesitaprospectiveindustrialenzyme.TheGeomicr
5、obiumsp.EMB2isolateisdepositedinMicrobialTypeCultureCollection,Chandigarh,IndiawithaccessionnumberMTCC10310.Introductiontion,immobilization,mutagenesisanddirectedevolutionhasProteasesarethelargestsellingindustrialenzymes.Theirsaleisbeenattemptedbyvariousresearchers[3,4].Acurrentshiftinprojectedtoi
6、ncreasefurtherinthecomingyearswithanticipatedapproachhasbeentoscreenthediversifiedmicrobialsourcesforapplicationsinproteinprocessing,peptidesynthesisanddetergenttheenzymesthatarenaturallystableandexhibithighactivitiesinformulations(http://www.prlog.org/10039621-enzymes-industry-thepresenceoforganic
7、solvents.forecasts-to-2010-2015)[1,2].Above-mentionedapplicationsneces-Halophilesseemtobeapromisingsourceinthiscontext[5–8].sitatetheproteasestobestableinsalts,organicsolventsandalkalineTheyinhabitsaline/hypersal
