the use of genetic markers for detecting dna polymorphism, genotype

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1、MolecularPhylogeneticsandEvolution47(2008)974–985ContentslistsavailableatScienceDirectMolecularPhylogeneticsandEvolutionjournalhomepage:www.elsevier.com/locate/ympevTheuseofgeneticmarkersfordetectingDNApolymorphism,genotypeidentificationandphylogeneticrelationshipsamongbananacult

2、ivarsL.Venkatachalam,R.V.Sreedhar,N.Bhagyalakshmi*PlantCellBiotechnologyDepartment,CentralFoodTechnologicalResearchInstitute,KRSRoad,Mysore,Karnataka570020,IndiaarticleinfoabstractArticlehistory:Geneticvariationsandrelationshipsamong21commerciallyimportantbananacultivarsofSouthI

3、ndiaReceived6July2007wereevaluatedusing50decamerRAPDprimersand12ISSRprimers.TheprimerswereselectedafterRevised4March2008apreliminaryscreeningofseveralsuchprimersfortheirabilitytoproduceclearandreproduciblepat-Accepted11March2008ternsofmultiplebands.Theanalysesresultedintheamplifi

4、cationoftotally641bandsof200–Availableonline20March20083100bp,ofwhich382bandswerepolymorphic,correspondingtonearly60%geneticdiversity.TheRAPDandISSRsurveysbetweenpairsof21cultivarsrevealed60.15%and56.73%ofpolymorphicbands,respec-Keywords:tively.Astronglinearrelationshipwasobserv

5、edbetweentheResolvingpower(Rp)oftheprimeranditsBananaabilitytodistinguishgenotypes.Basedonthesedata,ageneticsimilaritymatrixwasestablishedandaGeneticdiversityRAPDdendrogramforeachsetofprimerswasdevelopedbyUPGMA.ThegeneticsimilaritycoefficientsinRAPDISSRanalysisrangedfrom0.3177to0

6、.7818andinISSRanalysisfrom0.1800to0.8462.AfingerprintingkeyResolvingpowerwasgeneratedwherethepresence/absenceofspecificRAPD/ISSRbandswererecordedforeachcultivar.Jaccard’ssimilaritycoefficientThepresenceofaspecificRAPD(OPC-5800)bandwasobservedforanendemiccultivar—NanjanaguduRasabale(

7、NR).ThestudyresultedintheidentificationandmolecularclassificationofSouthIndianbananacultivarsofwhichRobustaandWilliamsareglobalandothershaveeitherlimitedgeographicaldistributionorpurelyendemictoSouthIndia.Agroupofeightcultivarswasidentifiedthatarehighlydistinctfromoneanother.Themem

8、bersofthisgroupmaybeusefulforgenerating2Xand4Xb