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1、ResearchArticleReceived:22October2010Revised:09January2011Accepted:14February2011PublishedonlineinWileyOnlineLibrary:7April2011(wileyonlinelibrary.com)DOI10.1002/jsfa.4397EvaluationofISSRmarkersforgeneticdiversityanalysisinAnatolianWaterBuffaloesIbrah
2、imAytekin,a∗FulyaOzdil,aUgurZulkadir,aSaimBoztepeaandVahdettinSariyelbAbstractBACKGROUND:Theutilisationofmolecularmarkershasincreasedinmolecularresearchrecently.Inter-simplesequencerepeat(ISSR)markersallowtheanalysisofgenomeswithoutpreliminarysequence
3、information,sincerandomprimersareused.ThepurposeofthepresentstudywastoevaluateISSRmarkersforassessingthegeneticdiversityofindigenousAnatolianWaterBuffaloesrearedinAfyon,KonyaandSivasprovincesofTurkey,withaviewtoconservationofthegeneresources.RESULTS:T
4、he11ISSRprimerschosenfortheanalysisrevealedatotalof110bands,ofwhich76(69.09%)werepolymorphic.Also,geneticsimilarity,polymorphicinformationcontent(PIC),resolvingpower(Rp)andmeanresolvingpower(Rp),heterozygosity(H)andShannonindex(I)werecalculatedas0.947
5、9–0.9562,0.35±0.20,2.73,0.27,0.18±0.07and0.28±0.11,respectively.CONCLUSION:TheISSRmarkerswerefoundtobepromisingforassessingthegeneticdiversityinbuffalopopulations.PotentialgeneticparameterssuchasPIC,Rp,Rp,HandIwereeffectivelyusedinthisstudy.c2011Soci
6、etyofChemicalIndustryKeywords:ISSRmarkers;AnatolianWaterBuffaloes;geneticdiversity;geneticparameters;resolvingpowerINTRODUCTIONpopulationandmilk/meatproductionhasdeclined57%andInvestigatingthepopulationstructureofaspeciesindangerof81%,respectively.16B
7、ecauseofthelowmilkandmeatyields,extinctioninordertoformulateefficientconservationstrategiesbuffalorearinghasbeenreducedindairyfarmingrecently.Inforthemisveryimportant.Inthepastdecade,inter-simpleordertoimprovetheproductivityofthesebuffaloesbymoleculars
8、equencerepeat(ISSR)markershavebeenusedtoinvestigatemethodsthereisanurgentneedfortheirgeneticcharacterisationthegeneticdiversityofnaturalpopulations.ISSRmarkersareandconservation.Animalgeneticresourcesaredepletingandthecharacterisedbydominantin
