DB43∕T 2321-2022 团头鲂繁育技术规程(湖南省)

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ICS67.080CCSB3143湖南省地方标准DB43/T2321—2022团头鲂繁育技术规程Technicalregulationsforbreedingofbluntsnoutbream2022-05-06发布2022-08-06实施湖南省市场监督管理局发布

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2DB43/T2321—2022目次前言························································································································Ⅲ1范围·····················································································································12规范性引用文件······································································································13术语和定义············································································································14环境与设施············································································································14.1水质···············································································································14.2亲鱼培育池······································································································14.3孵化设施·········································································································15亲鱼培育···············································································································25.1亲鱼···············································································································25.2水质管理·········································································································25.3饲养管理·········································································································26人工催产···············································································································26.1亲鱼的选择······································································································26.2催产激素·········································································································26.3注射剂量·········································································································26.4注射方法·········································································································26.5雌雄配比·········································································································26.6效用时间·········································································································26.7人工授精·········································································································37人工孵化···············································································································37.1孵化密度·········································································································37.2孵化管理·········································································································38鱼苗培育···············································································································39制种档案记录·········································································································3I

3DB43/T2321—2022II

4DB43/T2321—2022前言本文件按照GB/T1.1—2020《标准化工作导则第1部分：标准化文件的结构和起草规则》的规定起草。请注意本文件的某些内容可能涉及专利。本文件的发布机构不承担识别专利的责任。本文件由湖南省农业农村厅提出。本文件由湖南省农业标准化技术委员会归口。本文件起草单位：湖南师范大学、湖南省生物研究所、湖南省水产科学研究所。本文件主要起草人：肖亚梅、刘文彬、符文、彭亮跃、罗凯坤、刘锦辉、王冬武、何志刚、李金龙、曾国清、李绍明。III

5DB43/T2321—2022IV

6DB43/T2321—2022团头鲂繁育技术规程1范围本文件规定了团头鲂繁育的环境与设施、亲鱼培育、人工催产、人工孵化、鱼苗培育和制种档案记录的要求。本文件适用于团头鲂的繁育。2规范性引用文件下列文件中的内容通过文中的规范性引用而构成本文件必不可少的条款。其中，注日期的引用文件，仅该日期对应的版本适用于本文件；不注日期的引用文件，其最新版本（包括所有的修改单）适用于本文件。GB3838地表水环境质量标准GB/T5055青鱼、草鱼、鲢、鳙亲鱼GB11607渔业水质标准NY/T755绿色食品渔药使用准则NY/T5361无公害农产品淡水养殖产地环境条件SC/T1077渔用配合饲料通用技术要求DB43/T634畜禽水产养殖档案记录规范3术语和定义本文件没有需要界定的术语和定义。4环境与设施4.1水质应符合GB3838、GB11607的规定。4.2亲鱼培育池22宜为方形土池，面积1500m～2000m，池深2.5m～3m，池底平整，配置1.5kW叶轮式增氧机1台。4.3孵化设施4.3.1孵化池长4m、宽2m、深1.5m，配置粘卵板20～25块，底部安装喷水管、充气管。1

7DB43/T2321—20224.3.2粘卵板用不锈钢或铝材制成长120cm、宽65cm的框架，将40目纱布固定在框架上。5亲鱼培育5.1亲鱼经群体选育的雌雄团头鲂亲鱼分池培育，每亩池塘放养亲鱼300尾～500尾。放养前，用3％的氯化钠溶液浸泡消毒10min～15min。亲鱼培育应符合GB/T5055的规定。5.2水质管理水源水质应符合GB11607的规定，环境应符合NY/T5361的规定。每15d～20d换注一次新水，每次注水深度为15cm～20cm，保持水深1.5m～2.0m，保证溶氧量≥4.0mg/L。5.3饲养管理饲料以配合饲料为主，应符合SC/T1077的规定，粗蛋白含量≥30％。日投喂量为鱼体重的2％～5％，每日投喂2次～3次。6人工催产6.1亲鱼的选择每年5月上旬～6月中旬，繁殖水温为20℃～24℃。亲本要求色泽正常、健壮无病、光滑无伤、无畸形、肥满度适中。母本应选择Ⅱ龄以上，腹部膨大，卵巢轮廓明显，松软有弹性的个体，体重≥600g；父本（群体选育的雄性团头鲂）应选择Ⅱ龄以上，腹部相对较小，轻压腹部有乳白色精液流出的个体，体重≥500g。雌雄配比约为2∶1。6.2催产激素宜促黄体素释放激素类似物（LRH-A3）和绒毛膜促性腺激素（HCG）混合注射。6.3注射剂量雌鱼：LRH-A34μg/kg～6μg/kg，HCG300IU/kg～500IU/kg；雄鱼：LRH-A32μg/kg～3μg/kg，HCG200IU/kg～300IU/kg。用0.7％生理盐水配制注射。6.4注射方法采用一次注射催产，从胸鳍基部无鳞处倾斜45˚注入。6.5雌雄配比注射后亲鱼按照雌雄比例2∶1放入产卵池中待产。6.6效用时间在水温为18℃～24℃时，催产激素的效应时间为8h～14h。2

8DB43/T2321—20226.7人工授精亲鱼开始顺利产卵和产精时将亲鱼捞起，采用干法授精的方法进行人工授精：先将亲鱼体表水分擦干，将精卵同时挤入干燥的搪瓷盘内，用硬羽毛轻轻快速搅匀，使精卵充分混合。然后将受精卵均匀平22铺粘卵板上，密度为4颗/cm～6颗/cm。7人工孵化7.1孵化密度将铺有受精卵的粘卵板垂直并列悬挂在孵化池内，粘卵板底部距池底10cm～15cm，粘卵板间隔10cm～15cm。7.2孵化管理7.2.1消毒各种孵化工具在进孵化池前以及使用一次后均需重新消毒，药物使用应符合NY/T755的规定。7.2.2孵化用水33进水经60目纱布过滤,喷水管水压＞0.1Mpa，入池流量1T/h.m～2T/h.m，孵化早期水量可适当加大，鱼苗脱膜过程中水量调小，脱膜后再恢复。水温20℃～24℃，pH7.0～8.0。7.2.3增氧22充气管气压0.2kg/cm～0.7kg/cm，保持孵化水体溶氧≥5mg/Ｌ。7.2.4光照避免光线直射。8鱼苗培育孵化出苗后撤去粘卵板，鱼苗在孵化池中继续培养3d～5d，卵黄消失后投喂豆浆或轮虫，日投2次，鱼苗点腰平游即可出苗。9制种档案记录按照DB43/T634的规定执行。3