苦槛蓝叶的化学成分及其抑菌活性研究

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1、热带亚热带植物学报　2014，22(3)：307~313JournalofTropicalandSubtropicalBotany苦槛蓝叶的化学成分及其抑菌活性研究*叶慧娟,戴航,吴伦秀,郭育晖,谷文祥(华南农业大学理学院生物材料研究所，广州510642)摘要：为研究苦槛蓝(MyoporumbontioidesA.Gray)的化学成分，采用多种柱色谱技术从苦槛蓝叶中分离得到13个化合物，它们的结构分别鉴定为：野黑樱苷(1)、类叶升麻苷(2)、5,7-二羟基二氢黄酮(3)、3-O-β谷甾醇苷(4)、(3R)-oct-1-en-3-ol-O-β-D-glucopy

2、ranosyl-(1″→2′)-O-β-D-glucopyranoside(5)、7-甲氧基香橙素(6)、异樱花素(7)、匙叶桉油烯醇(8)、愈创木醇(9)、(1S,2R,5S,6R)-2,6-bis(5-methoxy-3,4-methylenedioxyphenyl)-3,7-dioxabicyclo[3.3.0]octane(10)、(1R,2S,5R,6S)-2-(4-hydroxy-3-methoxyphenyl)-6-(3,4-methylenedioxyphenyl)-3,7-dioxabicyclo[3.3.0]octane(11)、去甲基川陈

3、皮素(12)和3′,4′,5,6,7,8-六甲氧基黄酮(13)，其中化合物4、5、7~11为首次从苦槛蓝植物中分离得到。刃天青显色法测试部分化合物对大肠杆菌与金黄色葡萄球–1菌的抑制活性，结果表明，5,7-二羟基二氢黄酮(3)对大肠杆菌与金黄色葡萄球菌有一定的抑制作用，其MIC值为62.50μgmL。关键词：苦槛蓝叶；化学成分；抑菌活性doi:10.3969/j.issn.1005–3395.2014.03.013ChemicalConstituentsfromLeavesofMyoporumbontioidesandTheirBacteriostaticAc

4、tivities*YEHui-juan,DAIHang,WULun-xiu,GUOYu-hui,GUWen-xiang(InstituteofBiomaterial,CollegeofScience,SouthChinaAgriculturalUniversity,Guangzhou510642,China)Abstract:TostudythechemicalconstituentsinMyoporumbontioidesA.Gray,thirteencompoundswereisolatedfromtheleavesofM.bontioidesbyvari

5、ouschromatographytechniques.Onthebasisofspectraldata,theywereidentifiedasprunasin(1),acteoside(2),5,7-dihydroxyflavanone(3),anthemisol(4),(3R)-oct-1-en-3-ol-O-β-D-glucop-yranosyl-(1″→2″)-O-β-D-glucopyranoside(5),7-methoxyaromadenrin(6),5,7-dihydroxy-2-(4-methoxyphenyl)-chroman-4-one(

6、7),spainulenol(8),guaiol(9),(1S,2R,5S,6R)-2,6-bis(5-methoxy-3,4-methylenedioxyphenyl)-3,7-dioxabicyclo[3.3.0]octane(10),(1R,2S,5R,6S)-2-(4-hydroxy-3-methoxyphenyl)-6-(3,4-methylenedioxyphenyl)-3,7-dioxabicyclo[3.3.0]octane(11),demethylnobiletin(12)and2-(3,4-dimethoxyphenyl)-5,6,7,8-

7、tetra-methoxy-4H-chromen-4-one(13).Compounds4,5,and7–11wereobtainedfromM.bontioidesforthefirsttime.TheinhibitoryactivityagainstStaphyloccocusaureusandEscherichiacoilweredeterminedbytheuseofa96wellplateassay.Theresultsshowedthat5,7-dihydroxyflavone–1(3)possessedmodestlevelofantibacteri

8、alactivitywithMICva