新生儿缺氧缺血性脑病钙平衡紊乱与脑损伤的相关性研究论文

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1、新生儿缺氧缺血性脑病钙平衡紊乱与脑损伤的相关性研究论文.freelicencephalopathy,HIE)患儿红细胞内外钙浓度及红细胞膜上Ca2+-Mg2+-ATPase活性,研究所检测指标与脑损伤程度之间的关系,并探讨出现低钙血症时能否钙剂治疗,从而最大限度地提高HIE的疗效,减少死亡和伤残。方法采用流式细胞仪测定86例不同程度新生儿缺氧缺血性脑病患者红细胞内Ca2+浓度,以I-STAT1-Analyzer测定血浆游离钙,以化学比色法测定红细胞膜Ca2+-Mg2+-ATPase活性;以

2、30例足月新生儿作为对照组,均为羊水吸入和咽下综合征患者。将HIE组中部分患儿再分为补充钙剂组与未补充钙剂组,当补充钙剂疗程结束后再次检测各组相关指标。结果HIE急性期血浆游离钙浓度水平均明显低于对照组,(P0.05),红细胞内Ca2+浓度水平均明显高于对照组(P0.05),红细胞膜上Ca2+-Mg2+-ATPase活性明显低于对照组(P0.05);这些指标的变化与脑损伤的严重程度相关;在补充钙剂后红细胞膜上Ca2+-Mg2+-ATPase活性及红细胞内Ca2+浓度均与未补充钙剂组差异

3、无统计学意义(P0.05),.freeledetectiveindicatorsandtheneonatalhypoxic-ischemicencephalopathy(HIE)braininjury,bydetectingtheCa2+concentrationsinsideandoutsideoferythrocytesasembranesinchildrentreatmentcouldbeusedinthesituationofhypocalcemiaforthepurposeofimproving

4、theeffectofHIEmaximatilyanddecreasingmortalityanddisability.MethodsTheclinicalof86casesetry,plasmafreecalciumbyI-STAT1-AnalyzeranderythrocytemembranesCa2+-Mg2+-ATPaseactivitybychemicalassay.Asthecontrolgroup,30casesoffullterminfantsnioticfluid.TheHIEgro

5、upsupplement.Theaboveindicatorstreatment.ResultsInHIEacutephase,plasmafreeCa2+concentrationsembraneCa2+-Mg2+-ATPaseactivitysupplement,erythrocytemembraneCa2+-Mg2+-ATPaseactivityanderythrocyteintracellularfreeCa2+concentrationsgroup(P0.05),aconce

6、ntrationoffreecalciumionisoverloaded,plasmafreeCa2+concentrationanderythrocytemembraneCa2+-Mg2+-ATPaseactivityaredecreasedinHIE.Aftercalciumsupplement,erythrocyteintracellularfreeCa2+concentrationdidnotsignificantlyincrease.calciumsupplementisfeasib

7、leinHIEchildrenia.Butthisstudydidnotaddressoverloadduration.Therefore,short-termapplicationshouldbeusedaslongastheremissionofclinicalmanifestations.Andplasmafreecalciumisareferenceonly.【Keyicencephalopathy;Ca2+concentrationinsideandoutsideoferythrocytes;E

8、rythrocytemembraneCa2+-Mg2+-ATPaseactivity钙与新生儿缺氧缺血性脑病的关系已得到公认,1977年Nicholson第一次提出钙介导缺氧缺血后神经元死亡的学说1,1992年Schurr等在体外实验中发现Ca2+超载假说最能说明缺氧缺血性脑损伤后神经元死亡的机制,其后研究已证实缺氧缺血常引起细胞内钙超载,诸多实验都证实在缺氧造成的神经元损伤时,有细胞内