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1、黄海葵相关细菌抗菌活性菌株的筛选及其初步分类鉴定416中田抗生素杂志2011年6月第36卷第6期文章编号:1001—8689(2011)06—0416—05黄海葵相关细菌抗菌活性菌株的筛选及其初步分类鉴定刘祝祥黄苛肖怀东(植物资源保护与利用湖南省高校重点实验室,陈奇辉贺建武陈义光吉首大学生物资源与环境科学学院,吉首416000)摘要:目的对分离自硇洲岛黄海葵(Anthopleura口Drn删lc口)样品的126株细菌进行抗菌活性筛选和初步分类鉴定.方法以8种微生物(3株革兰阳性菌,3株革兰阴性菌
2、和2株真菌1作为指示菌,采用琼脂扩散法筛选抗菌活性菌株,采用基于l6SrRNA基因序列的系统发育分析和生物学特性研究对其中抗菌谱较广且抗菌活性稳定的菌株进行初步分类鉴定.结果67株菌具有抗菌活性,阳性率为53.2%,抗菌谱较J'且活性较强的9株,占受试菌株总数的7.1%.经过形态观察,生理生化特征及基于16SrRNA基因序列的系统发育分析表明,9株具有较强抗菌活性的菌株分别属于芽孢杆菌属(Bacillus1,盐芽孢杆菌属(Halobacillus),短杆菌属(Brachybacterium1,拟
3、诺卡菌属(Nocardiopsis1,盐单胞菌属(Halomonas),假交替单胞菌属(Pseudoalteromonas)~弧菌属(~brio)菌株.结论分离自中国南海的黄海葵相关微生物抗菌活性菌株具有丰富的类群多样性.关键词:黄海葵;细菌;抗菌活性筛选;系统发育分析;鉴定中图分类号:R378文献标识码:AScreeningandpreliminaryidentificationoftheantimierobialstrainsassociatedwithAnthopleuraxanthogr
4、ammicaLiuZhu-xiang,HuangKe,XiaoHuai—dong,ChenQi-hui,HeJian—WU,ChenYi?guang(KeyLaboratoryofPlantResourcesConservationandUtilizationofHunanProvince,CollegeofBiologicalResourceandEnvironmentScience,JishouUniversity,Jishou416000)Abstract0biectiveToinvest
5、igatetheScreeningandpreliminaryidentificationof126antimicrobialstrainsisolatedfromAnthopleuraxanthogrammicacollectedfromtheareaoftheNaozhouislandintheSouthChinaSea.MethodsTheantimicrobialactivityofthestrainsweretestedusingtheagardiffusionmethodemploy
6、ingeightindicatorstrainsincludingSarcinalutea,Staphylococcusaureus,Escherichiacoli,尸teusvulgar~,Bacillussubtilis,Candidaalbican,AsperillusnigerandEnterobacteraerogenes.Thepreliminaryidentificationofthestrainswereinvestigatedbyusingphylogeneticanalysi
7、sbasedon16SrRNAgenesequencecomparisons.ResultsTheresultsshowedthat67strainsoutofthe126strainsexhibitedantibacterialactivity.inwhich9strainsexhibitedstrongantibacterialactivity.Basedontheresultsofmorphologiccharacteristics.physiologicalandbiochemicalp
8、ropertiesandphylogeneticanalysisof16SrRNAgene,9strainsbelongedtothegeneraBacillus,Halobacillus,Brachybacterium,Nocardiopsislomonas.PseudoalteromonasandVibriorespectively.ConclusionTheresultspresentedhereshowedthatthereisabundantdiversityofpopulationo
