夏至草醇提物干预实验性弥散性血管内凝血大鼠肺损伤的实验研究.doc

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1、夏至草醇提物干预实验性弥散性血管内凝血大鼠肺损伤的实验研宄作者：晁怀宇，张玉平，刘正泉，雷慧，杜舒婷，姜华【摘要】目的观察夏至草醇提物对高分子右旋糖酐致弥散性血管内凝血大鼠肺损伤的干预作用。方法Wistar雄性大鼠20只，随机分为夏至草组、模型组和对照组。静脉推注10%Dextran500复制DIC模型。6min后，夏至草组自颈静脉缓慢推注夏至草醇提物，其它两组以等量生理盐水代替。40min后，制备肺组织匀浆，观察肺匀浆自由基指标MDA含量及SOD活性、N0含量及N0S活性的变化。结果与对照组相比，模型组肺组织匀浆MDA、NO含量及NOS活性显著升高，SOD活性降低；夏至草组肺组织

2、匀浆MDA、N0含量及NOS活性显著低于模型组，SOD活性升高，各指标与对照组比较无统计学意义。结论夏至草醇提物减轻Dextran500致DIC大鼠肺损伤的机制与降低自由基损伤、降低N0的生成与释放有关。【关键词】夏至草；弥散性血管内凝血；肺损伤；自由基；一氧Abstract:ObjectiveToobservetheeffectsofethanolextractfrominstlunginjuryofdicuclarcoagulation(MrrubiumincisumagasseminatedintravasDIC)ratsinducedbyDextranmaleWistarr

3、atswererandomlydividedintothreegroups:Mrrubiumincisumgroup(n=8),modelgroup(n=6)andcontrolgroup(n=6)•TheDICmodelwasestablishedbyintravenousinjectionofDextran500(10m1/kg•bw),andDextran500wasreplacedbyquivalentnormalsalineincontrolgroup.6minuteslater,ethanolextractfromMrrubiumincisumwasinfused(5g

4、/ml,6g/kg•w)；inothergroupsethanolextractfrom^Irrubiumincisumwasreplacedbynormalsaline.After40min,thelungwastakenout,the10%tissuehomogenatewasprepared,andthefreeradicalindexesofMDAcontentandSODactivity,NOcontentandNOSactivitywerecontentsofMDAandNOandNOSactivityof1unghomogenateinmodelgroupweresi

5、gnificantlyincreased,andSODactivityweresignificantlylowerthanthatincontrolgroup.ThecontentsofMDAandNOandNOSactivityoflunghomogenateinMrrubiumincisumgroupweresignificantlylowered,andSODactivityweresignifiomparedwithmodelgristicaldifferencebsumgroupandcontrolhanolextractfromMrrkedlyabatelunginjr

6、an500anditsmechancantlyincreasedascoup.TherewasnostatetweenMrrubiumincigroup.ConclusionEtrubiumincisumcanmauryofDICratsbyDextismmayberelatedtoscavengingfreeradicalanddecreasingNOrelease.Keywords：Mrrubiumincisum；Disseminatedintravascuclarcoagulation(DIC)；Dextran500；Lunginjury；Freeradical；Nitric

7、oxide弥散性血管内凝血是临床常见的危重病理过程，在此病理过程中所形成的微血栓、以及出血后引起的低血容量、微循环障碍等因素，是导致危重患者出现肺损伤甚至多个器官功能障碍的主要因素之一。因此，寻求合理的治疗措施防止DIC引起的肺损伤成为临床研究的重点内容。研究表明，高分子右旋糖酐在导致大鼠DIC及血液流变性异常的同时，可引起大鼠肝、肾、肺等多个器官组织形态学损伤，组织学观察可见红细胞淤滞、微血栓及细胞坏死等［1］;夏至草可明显改善血淤大鼠的血液和淋巴微循环障碍［2，3