il17a对gmcsf诱导的骨髓树突状细胞的影响

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1、天津医科大学硕士学位论文costimulatorymoleculesCD40，CD86andMHCIIbyinductionGM．CSEAtthesametime．ithasadose．dependent．InordertoconfirmtheeffectofIL．17AtomllydifferentiatedBMDC，weaddedrmlL．17(50，100or500ng／m1)toBMDCinducedbyGM．CSFfor8days．thencontinueculture36hours．Compared、析tIlN0rmlL．17

2、A，BMDCsurfacecostimulatorymoleculesCD40，CD80，CD86，MHCIIandthecytokinessecretioninthesupernatantIL．12p40andIL．10werenotsignificantlychangedofeachgroup．ItshowsthatIL一17AhasnofunctionofstimulatingfullydifferentiatedBMDCtomature．ButIL．17Aup．regulatestheexpressionofcostimulato

3、rymoleculesonDCandthesecretionofIL．12P40andIL．1OintheprocessofGM．CSF．induced．BMDCwithLPS．Conclusions：T11iSexperimentusesthemethodofGM．CSF．inducingandLPSstimulizationsuccessfullyculturedandamplificationDC．IL．17ApromotesthedevelopmentofDCprogenitorsphenotypewhichisinducedby

4、GM-CSF．IL一17AalonehasliRleinfluenceonthedifferentiationofDC．IL．17Aup．regulatestheexpressionofDCcostimulatorymoleculesincludingCD40．CD80．CD86andMHC11whichaleexpressedonmatureDCstimulatedbyLPS．Italsoup—regulatethelevelofIL一12p40andIL一10secretedbyDCinducedbyLPS．Thesefindings

5、suggestthatIL一17AplaysanimportantroleinpromotingDCdifferentiation．Keywords：BMDCIL一17AGM—CSFDCcostimulatorymoleculeIL··12p40IL·-10IV天津医科大学硕士学位论文目录中文摘要⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯IAbstract·······················································································

6、II符号说明⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯VI前言⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯”⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯·1月IJ舌⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯”⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯。l研究现状、成果⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯2研究目的、方法⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯3对象和方法⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯41实验材料⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯41．1主要试剂⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯4

7、1．2主要仪器和设备⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯51．3主要试剂的配制⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯51．4实验动物⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯72实验方法⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯72．1小鼠BMDC的分离和培养⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯72．2CDllc免疫磁珠分离提纯成熟BMDC⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯82．3流式细胞术检测成熟BMDC表面分子⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯82．4ELISA检测细胞因子分泌⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯

8、⋯92．5统计分析⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯10结果⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯11l小鼠骨髓来源的DC体外扩增方法的建立⋯⋯⋯⋯⋯⋯⋯