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1、作物学报ACTAAGRONOMICASINICA2008,34(7):1199−1205http://www.chinacrops.org/zwxb/ISSN0496-3490;CODENTSHPA9E-mail:xbzw@chinajournal.net.cnDOI:10.3724/SP.J.1006.2008.01199陆地棉遗传图谱构建及产量和纤维品质性状QTL定位*陈利张正圣胡美纯王威张建刘大军郑靓郑风敏马靖(西南大学农学与生物科技学院/农业部生物技术与作物品质改良重点实验室,重庆400716)摘要:利用3458对SSR
2、引物筛选陆地棉中棉所35和渝棉1号间的多态性引物,获得173对。以多态性引物检测(渝棉1号×中棉所35)F2群体180个单株的标记基因型,共获得178个标记位点。构建的遗传连锁图谱包括148个标记,36个连锁群,总长1309.2cM,标记间平均距离8.8cM,覆盖棉花基因组的29.5%。36个连锁群中的28个分别被定位于20条染色体,8个连锁群未定位于染色体。以渝棉1号×中棉所35的F2、F2:3群体的产量、纤维品质性状鉴定结果,利用区间作图方法,检测到4个产量性状QTL,即2个衣分(LP)、1个铃重(BW)、1个籽指(SD);
3、5个纤维品质性状QTL,即1个纤维长度(FL)、2个纤维比强度(FS)和2个纤维细度(FF)。LP1、BW、SD、FL和FS1被定位于第7染色体,LP2、FS2、FF1和FF2被分别位于第15、21、9和20染色体。5个纤维品质QTL的有利等位基因均来源于渝棉1号。关键词:陆地棉;遗传连锁图谱;产量;纤维品质;QTLGeneticLinkageMapConstructionandQTLMappingforYieldandFi-berQualityinUplandCotton(GossypiumhirsutumL.)*CHENLi
4、,ZHANGZheng-Sheng,HUMei-Chun,WANGWei,ZHANGJian,LIUDa-Jun,ZHENGJing,ZHENGFeng-Min,andMAJing(KeyLaboratoryofBiotechnology&CropQualityImprovement,MinistryofAgriculture/CollegeofAgronomy&Biotechnology,SouthwestUni-versity,Chongqing400716,China)Abstract:Cottonistheworld’s
5、mostimportantnaturaltextilefiber.Withthechangesinspinningtechnology,theimprovementofcottonfiberqualityisbecomingextremelyimportant.ThecombinationofconventionalbreedingtechniqueswithMAS(mo-lecular-assistedselection)willbecomeabasicapproachforcottonfiberimprovement.Int
6、hepresentstudy,3458SSRprimerpairswereusedtoscreenthepolymorphicprimersbetweenZhong35andYumian1,and173polymorphicprimerpairswereob-tained.Thepolymorphicprimerpairswereusedtogenotypethe180individualplantsofF2populationderivedfrom(Zhong35×Yumian1),and178lociwereobtained
7、.Ageneticlinkagemapwith148SSRlociand36linkagegroupswasconstructed,andthelinkagemapcoveredawholelengthof1309.2cMwithanaverageintervalof8.8cMbetweentwomarkers,accountingfor29.5%ofthecottongenome.28outof36linkagegroupswerelocatedon20chromosomes,and8linkagegroupswerenotl
8、ocatedonanychromosomes.Thedataofyieldandfiberqualitiesofthe180F2plantsandF2:3familylineswereusedtodetectQTLsbyintervalmapping,and9Q
