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1、4.脑组织切片HE染色显示：B组组织明显水肿，神经细胞体积增大，空泡变性，细胞间隙增大；E组神经细胞数量多,胞浆丰富,细胞受损程度在各组中最轻,低倍镜下可见散在的小软化灶，其余组织结构相对较好。结论1．骨髓间充质干细胞和血管内皮祖细胞移植治疗缺血性脑损伤可以促进神经功能的恢复，联合移植效果优于单细胞移植。2．骨髓间充质干细胞和血管内皮祖细胞移植治疗缺血性脑损伤的作用机制之一可能是通过上调Bcl-2蛋白表达,下调Bax蛋白表达,减少神经细胞凋亡。关键词骨髓间充质干细胞；血管内皮祖细胞；大脑中动脉梗塞；移植；Bcl-2；Bax硕士研究生：陈芬芳导师：汤
2、永红教授3EffectsofBMSCsandEPCstransplantationandexpressionsofBcl-2,Baxinratcerebralischemia-reperfusiontissueAbstractObjective：Thisstudyusedbonemarrowstromalcellsbonemarrowstromalcells,(BMSCs),endothelialprogenitorcells(endothelialprogenitorcells,EPCs)transplantsIntervenedtheratbr
3、ainischemia-reperfusionmode.ToinvestigatetheexpressionsofBcl-2,Baxandtheeffectonbonemarrowstromalcells(BMSCs)andendothelialprogenitorcells(EPCs)transplantationfortreatingcerebralischemiainrats.Methods:Ratmiddlecerebralarteryocclusion(MCAO)modelwasestablished.Atotalof100SDratsw
4、eredividedintoshamoperationgroup(Agroup),operationnotreatinggroup(Bgroup),BMSCs(tailvein)group,EPCSgroupandBMSCs+EPCScomplexgroupatrandom.Agroupofanesthesia,onlyisolatedtothecarotidartery,suturingtheskinthatisnotanytreated.BgroupisMCAOratmodel.Ischemiareperfusion2h,7dayafterpr
5、eparationofthemodelmade,grouptraining,respectively,BMSCs,EPCsdissolvedinphosphatebuffer66solution,cellnumberwasadjustedto2×10/L.Cgroupistransplanted2×10/L6BMSCsintotheMCAOtailvein.Dgroupistransplanted2×10/LEPCsintotheMCAO66tailvein.Egroupistransplanted2×10/LEPCsand2×10/LBMSCsi
6、ntotheMCAOtailvein.Transplantationineachgroupequaltothetotalvolumeofliquid.After7daysofreperfusionwereobservedneurologicscore,HEstaining,bcl-2andbax.Results:1:Neurologicscore:groupA,B,C,D,Ewas0，3.92±0.31，2.67±0.43，3.01±0.45分，1.92±0.25。repectively.GroupBwassignificantlyhigherth
7、angroupC,andgroupDshowedastatisticallysignificantdifference（P<0.05）,whilegroupEshowedastatisticallysignificantdifferencecomparedwithgroupCandgroupD.2：ImmunohistochemicalstainingwasusedtodetecttheexpressionofBcl-2:The4expressionlevelofBcl-2ofgroupA,B,C,D,Eis:8.69±10.14，12.59±4.
8、26，33.37±6.51，22.28±7.18，45.67±5.02。groupEwashighestandshowed
