中药何首乌饮抗大鼠卵巢组织衰老的机理[1]

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1、第39卷第2期解剖学报Vol139,No122008年4月ACTAANATOMICASINICAApr.2008·187·中药何首乌饮抗大鼠卵巢组织衰老的机理1,23413122张娜李亚丽牛嗣云高福禄陈雪郭丽娜赵素英(11河北医科大学组织学胚胎学教研室,石家庄050017;21河北医科大学第四医院生殖医学科,石家庄050011;31河北省人民医院三优中心,石家庄050071;41承德医学院基础医学研究所,承德067000)[摘要]目的探讨何首乌饮抗大鼠卵巢组织衰老的机理。方法36只SD大鼠随机分为3

2、组:正常组、模型组、预防组。采用D2半乳糖连续腹腔注射法制造雌性大鼠亚急性衰老模型,预防组在造模的同时以中药方剂何ARF首乌饮灌胃。应用逆转录聚合酶连反应(RT2PCR)方法检测各组大鼠卵巢组织p53、p19、Rb及p16基因的表达,用Westernblotting方法检测各组大鼠卵巢组织Rb及p16蛋白的表达情况。结果何首乌饮明显抑制了衰老大鼠ARF卵巢组织中p53、p19、Rb以及p16基因表达的上调趋势(P<0105),且明显降低了衰老大鼠卵巢组织中Rb及p16ARF蛋白的表达(P<0105)

3、。结论何首乌饮抑制衰老途径中关键的调节因子p53、p19、Rb及p16的表达,从而起到抗大鼠性腺衰老的作用。[关键词]何首乌饮;衰老;p53;Rb;逆转录聚合酶连反应;免疫印迹法;大鼠[中图分类号]Q9523,R21217[文献标志码]A[文章编号]052921356(2008)022187STUDYONANTI2AGINGINTHEOVARYOFTHEAGINGMODELRATBYTRADITIONALCHINESEMEDICINEHESHOUWUYINZHANGNa1,2,LIYa2li3,NI

4、USi2yun4,GAOFu2lu13,CHENXue1,GUOLi2na2,ZHAOSu2ying2(11DepartmentofHistologyandEmbryologyHebeiMedicalUniversity,Shijiazhuang050017,China;21DepartmentofReproductiveMedicine,theForthHospitalofHebeiMedicalUniversity,Shijiazhuang050011,China;31CenterofHealt

5、hyBirth,ExcellentGrowthandEducationthePeopleHospitalofHebeiProvince,Shijiazhuan050071,China;41InstituteofBasicMedicalSciences,theHospitalofChengdeMedicalCollege,Chengde067000,China)[Abstract]ObjectiveTofindthemechanismoftraditionalChinesemedicineHeshou

6、wuyin(HSWY)inanti2aginginovaryoftheagingmodelrats,soastoprovidetheoreticalandexperimentalevidencetoanti2aginginfemalesexualgland.MethodsThirty2sixratsweredividedintothreegroupsatrandom:normalgroup,agingmodelgroup,preventaginggroup.Ratstheinpreventaging

7、groupweresubdividedintofourgroups:HSWYlowdosagegroup,HSWYmediumdosagegroup,HSWYhighdosagegroupandHeshouwan(HSWW)group.Thesub2acutelyagingmodelratswereinducedbyD2galactose,andratsinthepreventaginggroupwerereceivedintragastricadministrationofHSWYandHSWWw

8、ithD2galactoseintraperionealinjection.TheARFgeneexpressionsofp53,p19,Rbandp16weredetectedintheovariesofeachgroupwithreversetranscriptionpolymerasechainreaction,andtheproteinexpressionsofRbandp16intheovarieswithWesternblotting.ResultsThe