溃疡愈药散对糖尿病足溃疡动物模型的疗效观察

《溃疡愈药散对糖尿病足溃疡动物模型的疗效观察》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、目录中文摘要···················································1英文摘要···················································3弓l言·······················----·························6正文···················-································91实验材料和方法·························

2、··············---91．1实验对象····-············································91．2动物饲养条件······························-----··········91．3主要试剂、药品和设备·························-············91．4实验方法···················--··--·······················102实验结果····---·······

3、··································153讨论················································244存在的问题和展望···············--··---················34结论·························-························37参考文献····---···········································38附录·--·-···

4、··········································41综述·······----·······································43刭【谢··········-·····································j·55攻读学位期间发表的学术论文目录·······················56作者声明···············································-·-57个人简历·-···

5、···································-·········58中文摘要目的：将经验药方溃疡愈药散运用于糖尿病足／溃疡动物模型上，并观察疗效，为临床应用提供理论依据。方法：(1)将40只大鼠随机分成空白、对照、实验三组，制备糖尿病溃疡大鼠模型，所有实验大鼠在实验过程中均适时进行血糖及体重的测量。(2)三组动物均予注射普通胰岛素基础处理，进行降糖治疗，将血糖控制在8retool／L左右。空白组：常规冲洗(用0．9％氯化钠注射液)+75％乙醇消毒液+抗感染药物(根据药敏培养选择)；对照

6、组：常规冲洗(药物同空白组)+溃疡愈药散外敷+抗感染药物(同空白组)；实验组：常规冲洗(药物同空白组、对照组)+溃疡愈药散外敷(药物剂量同对照组)，1次／日。(3)每次给药后用无菌纱布绷带包扎，给药时间为15天。(4)分别于治疗前、治疗5天后、15天后处死动物并取样。(5)各组于每个时间点拍照片，计算并比较创面愈合率。(6)各组于每个时间点取分泌物送检进行细菌学培养，并做药敏实验，对比治疗前后细菌增减变化。(7)各组于每个时间点取少量组织(包括结痂物)送检进行细胞学分析，包括HE染色，免疫组织化学CD68标记巨

7、噬细胞，观察皮肤及皮下组织的形态学变化。结果：(1)实验组创面愈合率(i_-／-s，％)在敷用溃疡愈药散第5天、第15天分别为37．933±4．46、98．29，对照组分别为37．13±2．78、98．09，两组相比相差不大，均优于空白组的26．59±5．5、96．17，经统计学分析第5天实验组与对照组分别与空白组相比，尺o．05，有统计学差异，第15天各组两两相比均无统计学意义(／bO．05)。(2)细菌学培养和药敏实验结果，糖尿病足／溃疡动物模型创面主要为金黄色葡萄糖菌感染，敷药5天，培养出细菌比例稍有增加

8、，敷药15天后各组均未培养出真菌，为正常菌群生长。(3)HE染色观察结果，实验、对照组切片组织学显微观察显示创面愈合情况优于空白组。(4)免疫组织化学CD68标记巨噬细胞结果观察，创面愈合开始时，巨噬细胞逐渐增多，实验组、对照组更为明显。愈合后期，几乎未见巨噬细胞。结论：运用溃疡愈药散外敷，可抑制细菌生长，减少药物的毒副作用，促进创面愈合，防止病情恶化，降低截肢率，为治疗糖尿病足／溃疡