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1、蚌埠医学院学报2008年7月第33卷第4期379[文章编号]10002200(2008)04037904基础医学+++正常人外周血自然杀伤T细胞(CD3CD16CD56)比例姚春艳,金齐力,姜丽娜,唐洁,李柏青+++[摘要]目的:探讨正常人外周血中自然杀伤T(NKT)细胞(CD3CD16CD56)比例的95%参考值范围。方法:取141名正常成年人和30例肺癌患者外周血,直接双色荧光抗体染色,溶血洗涤后用流式细胞仪检测NKT细胞和T细胞各亚群的数量。+++分析获取的结果以百分位数计算95%的正常值范围。结果:141
2、名正常人外周血NKT细胞(CD3CD16CD56)的百分率平均数为4.16%,标准差为4.13%,以百分位数法获得95%参考值范围为(0.78~11.71)%;18~30岁组、31~50岁组和51~+++70岁组的NKT细胞(CD3CD16CD56)比例的平均数分别为4.64%,3.91%和3.57%,差异无统计学意义(P>0.05);30++++例肺癌患者组与正常组该项指标的差异亦无统计学意义(P>0.05)。同时检测到正常人CD3细胞、CD3CD4细胞、CD3+++++-++CD8细胞、CD3CD4/CD3CD8比值以及
3、NK细胞(CD3CD16CD56)的比例均与大多数报道的正常值相似。结论:正+++常人外周血中NKT细胞(CD3CD16CD56)比例的95%参考值范围是(0.78~11.71)%,平均数为4.16%,中位数为2.97%,虽然NKT细胞比例随年龄增加而减少,但两者之间无直线相关关系。未发现肺癌组患者NKT细胞比例与正常人有显著性差异。[关键词]淋巴细胞;杀伤细胞,自然;流式细胞术;血细胞计数[中国图书资料分类法分类号]R392.1[文献标识码]A+++PercentageofnaturalkillerTcells(CD
4、3CD16CD56)inperipheralbloodofhealthyadultsdetectedYAOChunyan,JINQil,iJIANGLina,TANGJie,LIBaiqing(DepartmentofImmunology,BengbuMedicalCollege;AnhuiKeyLaboratoryofInfectionandImmunityatBengbuMedicalCollege,BengbuAnhui233030,China)+++[Abstract]Objective:Todetectth
5、eproportionofnaturalkillerT(NKT)cells(CD3CD16CD56)inperipheralbloodamonghealthyadultsbyflowcytometry.Methods:Thewholeperipheralbloodof141healthyadultsand30patientswithlungcancerwerecollectedandstainedwithdoublecoloredfluorescentantibodies,followedbylysingredbloodce
6、llsandwashingcells.ThepercentagesofNKTcells,TlymphocytesandTcellsubsetsweremeasuredandanalyzedbyflowcytometry.The95%referencerangewascalculatedby+++percentile.Results:TheaverageofpercentageNKT(CD3CD16CD56)cellsinthe141healthyadultsperipheralbloodwas4.16%;95%referen
7、cerangebypercentilewasfrom0.78%to11.71%.TheaveragesofNKTcellsintheagegroupsof18-30year,31-50yearand51-70yearwere4.64%,3.91%,and3.57%,respectively.Therewasnosignificantdifferenceamongthethreegroups(P>0.05).TheaveragepercentageofNKTcellsintheperipheralbloodofpatie
8、ntswithlungcancerdidnotchangesignificantly,comparedtothenormalgroup.Inaddition,theproportionsofTcellsubsetsandNKcellsmeasuredinthisstudywere+++wi
