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1、网络出版时间：2017-01-1017:43:54网络出版地址：http://www.cnki.net/kcms/detail/44.1620.TS.20170110.1743.076.html现代食品科技ModernFoodScienceandTechnology2017,Vol.33,No.4DNJ对正常小鼠脂代谢影响及作用机理初探1112王玲，曾艺涛，丁晓雯，黄先智（1.西南大学食品科学学院，重庆400716）（2.家蚕基因组生物学国家重点实验室，重庆400716）摘要：本文为探索DNJ对脂代谢的影响及作用机理，给正
2、常小鼠灌胃DNJ40d后，用试剂盒测定各实验动物脂代谢相关生化指标、酶活及抗氧化指标。结果显示与阴性对照组比较，8.0mg/kg·bw/d剂量DNJ使雌、雄小鼠的体重增加率分别降低59.40%和9.15%；腹腔脂肪系数降低38.30%和15.18%；肝脏脂肪含量降低18.42%和36.66%；血清TC降低27.40%和28.57%，TG降低0.89%和15.03%，HDL增加42.19%和6.80%；肝脏SOD活性上升29.24%和25.17%，GSH增加16.29%和15.00%，8-isoprostane下降18.53
3、%和15.19%；雌鼠FAS、ACO活性下降14.02%和9.67%，雄鼠ACO活性上升21.63%。说明适量DNJ主要通过抑制雌鼠脂肪酸合成、加速雄鼠脂肪酸氧化来减少脂肪蓄积，且对雌鼠具有更好的预防肥胖作用；同时可增强小鼠机体抗氧化能力，预防脂代谢异常。关键词：DNJ；脂代谢；酶活；抗氧化EffectandMechanismof1-DeoxynojirimycinonLipidMetabolisminNormalMice1112WANGLing,ZENGYi-tao,DINGXiao-wen,HUANGXian-zhi(
4、1.CollegeofFoodScience,SouthwestUniversity,Chongqing400716,China)(2.StateKeyLabofSilkwormGenomeBiology,Chongqing400716,China)Abstract:Toexploretheeffectandmechanismof1-Deoxynojirimycinonlipidmetabolisminnormalrats,wegaveDNJfor40days,andthenwedeterminedthebiochemic
5、alindicatorsoflipidmetabolismandtheactivityofenzymesandtheantioxidantindexsbyELISAkit.Comparedwithnegativecontrolgroup,8.0mg/kg.bw/ddosageofDNJhadthebesteffectonpreventingobesity.Theratesofweightgainweredecreasedby59.40%(female)and9.15%(male)respectively,abdominal
6、fatfatorsweredecreasedby38.30%(female)and15.18%(male)respectively,coefficientsofliverfatweredecreasedby18.42%(female)and36.66%(male)respectively,contentsofTCinserumweredecreasedby27.40%(female)and28.57%(male)respectively,contentsofTGinserumweredecreasedby0.89%(fem
7、ale)and15.03%(male)respectively,contentsofHDLinserumwereincreasedby42.19%(female)and6.80%(male)respectively,activitiesofSODinliverwereincreasedby29.24%(female)and25.17%(male)respectively,contentsofGSHinliverwereincreasedby16.29%(female)and15.00%(male)respectively,
8、contentsof8-isoprostaneinliverweredecreasedby18.53%(female)and15.19%(male)respectively,activitiesofFASandACOinfemalemice’liverwereincreasedby14.02%and9.
