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1、VIROLOGY78,500-510(1977)TheRNAofHumanCoronavirusOC-43GREGORYA.TANNOCK’ANDJOHNC.HIERHOLZERRespiratoryVirologyBranch,VirologyDivision,BureauofLaboratories,CenterforDiseaseControl,PublicHealthService,U.S.DepartmentofHealth,Education,andWelfare,Atlanta,Georgia30333Accepte
2、dFebruary3,1977AhomogeneousRNAcomplexwithasedimentationcoefficientof70Sandanappar-entmolecularweightofapproximately6.1x10’wasreleasedfrompurified32P-labeled,mouse-brain-derivedOC-43virusaftertreatmentwith1%sodiumdodecylsulfate(SDS)for15minat23”.Thecomplexwashighlysusc
3、eptibletoheat,releasing4SRNAfragmentsat37”andbreakingdowntofragmentsof4-70Sat60”;itwasalsodegradedbycentrifugationthroughdimethylsulfoxidegradients.UnliketobaccomosaicvirusorRoussarcomavirusRNA,OC-43RNApreparedbyextractionwithphenol-SDSorphenol-chloroformdegradedintoa
4、rangeoffragmentswithcoefficientsof15-55S;4SRNAwasalsopresentasaminorcomponent.Thissuggeststhat(a)extensivenickingofalargeRNAmoleculehasoccurredduringviralgrowth,duetoribonucleaseswhichareinactivatedduringphenolextractions;(b)heterogeneityforOC-43RNAisnotduetointernalr
5、ibonucleaseactivityandfragmentsareheldtogetherbynoncovalentlinkagesmuchweakerthanthosepresentinthe70SretroviralRNAcomplex,orbysmallproteins;or,mostprobably,(clacombinationofextensivenickingandweaknoncova-lentlinkagesresultsintheheterogeneousdenaturationproducts.INTROD
6、UCTIONscribedIBVRNA,afteritsreleasefromTheCoronaviridaeweredescribedasavirionsbySDSlysis,asasinglespeciesseparatetaxonomicgroupbyAlmeidaethavingamolecularweightof9.0xlo6andal.(1968)but,despitetheirubiquityandasedimentationcoefficientof50S.Theyeconomicimportance,fewbio
7、physicalreported,butwithoutsupportingevidence,characterizationstudieshavebeenre-thatbyphenolextractionarangeofRNAported.TheRNAofavianinfectiousbron-fragmentscouldbeobtainedwhichwaschitisvirus(IBV)wasfirstdescribedbysimilartothatTannock(1973)described.Tannock(1973),who
8、obtainedfrompuri-Garwesetal.(1975)examinedtheRNAsfiedvirionsahighlyheterogeneousarrayoftwoporcinecoronaviruses,transmissi-of
