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1、JournalofGeneralVirology(1995),76,431~435.PrintedinGreatBritain431Sequenceandexpressionofthens2proteingeneofhumancoronavirus0C43PatrickLabont6,SamirMounirandPierreJ.Talbot*VirologyResearchCenter,InstitutArmand-Frappier,UniversitdduQudbec,531boulevarddesPrairies,Laval,Qudbe
2、c,CanadaH7N4Z3Thecompletenucleotidesequenceofthens2geneofthatoftheMebusstrainofbovinecoronavirus(BCV).humancoronavirusOC43(HCV-OC43)wasdeter-However,astretchofnineconsecutiveaminoacidsnearmined.SequenceanalysisrevealedanopenreadingtheCterminusiscompletelydifferent,causingi
3、ttobeframethatcouldencodeaproteinof278aminoacids,veryhydrophilic,whichcontrastswiththehydrophobicwithanestimatedmolecularmassof32.2kDa.SixnatureofthisregioninBCV.Asshownbyimmuno-potentialphosphorylationsitesarepresentbutnositesfluorescencewithamonospecificantiserum,thens2o
4、fN-glycosylationwerefound.TheaminoacidsequenceproteinwasexpressedinthecytoplasmofHCV-OC43-oftheHCV-OC43ns2proteinshows92%identitywithinfectedHRT-18cells.CoronavirusesareenvelopedvirusesthatcontainaHumancoronavirusOC43comprisesfourorfivesingle-strandedpositivesenseRNAgenome
5、of27tomajorstructuralproteins:apeplomer(S)glycoprotein,31kb(Boursnelletal.,1987;Leeetal.,1991).Theanhaemagglutinin-esterase(HE)glycoprotein,anucleo-genomicRNAencodessixtoeightcappedandpoly-capsid(N)phosphoproteinandamembrane(M)adenylatedsubgenomicmRNAsthatarearrangedinagly
6、coprotein(Mounir&Talbot,1992,1993a;ZhangetT-coterminalnestedsetstructure.EachmRNApossessesal.,1992;Kamahoraetal.,1989),aswellasapredictedacommon5'endleadersequencederivedfromthe5'endsmallmembraneprotein(sM)(Mounir&Talbot,1993b),ofthegenomicRNA.Ithasbeensuggestedthattheappa
7、rentlysimilartotheonepreviouslyidentifiedininteractionofthe3'endoftheleadersequencewiththevirionsofinfectiousbronchitisvirus(IBV)andTGEVfull-lengthminusstrandgenomicRNA,attheconsensus(Liu&Inglis,1991,Godetetal.,1992).Inadditiontointergenicsequences,initiatesthesynthesisofs
8、ub-thesestructuralproteins,HCV-OC43possessesseveralgenomicmRNAsofdiverselengths(Lai,1990)
