人脐血间充质干细胞源性心肌细胞的诱导分化研究

《人脐血间充质干细胞源性心肌细胞的诱导分化研究》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、国际心血管病杂志2010年9月第37堂箜塑!!塑!!兰望!!竺竺：!：!!!：·315·人脐血间充质干细胞源性心肌细胞的诱导分化研究杨水祥黄景玲【摘要】目的：探讨体外人脐血间充质干细胞(hMSCs)诱导分化成心肌细胞的可行性和最佳方法。方法：收集健康产妇脐血细胞，分离单个核细胞，从中进一步分离间充质干细胞，传代培养至第三代，应用免疫荧光流式细胞仪标记MSCs特异性抗原CD34，CD44和CD90。5-氮(杂)胞苷(5一aza)诱导分化4周后，免疫组织化学染色和RTPCR分别检测心肌细胞标志物肌钙蛋白I，GATA4和p一肌球蛋

2、白重链。结果：脐血源性MSCs经5-aza诱导分化后，呈现成纤维细胞样形态和克隆增殖特点。免疫分型与骨髓来源MSCs一致，且免疫组织化学染色和RT-PCR可检测到肌钙蛋白I、GATA4和一肌球蛋白重链的表达。结论：脐血源性MSCs能够被诱导分化成心肌样细胞，可成为干细胞移植的细胞来源。【关键词】间充质干细胞；脐血细胞；5-氮(杂)胞苷；心肌细胞DOI：10．3969／j．issn．1673—6583．2010．04．019HumanMSCsfromUCBcanbeintroducedtodifferentiateintoca

3、rdiomyocytesinvitroYangShui—xiangHuangJing—ling．1．DepartmentofCardiology，BeijingShijitanHospital，Beijing100038，China；2．DepartmentofCardiology，QinghaiProvinceCardiovascularFacultyHospital，Xining，Qinghai810012，China[Abstract]Objective：Inordertodetectthepossibilityand

4、thebestwayformesenchymalsterncells(MSCs)fromumbilicaIcordblood(UCB)tobeintroduceddifferentiati0nintocardiomyocytesinvitro．Methods：Theumbilicalcordbloodwascollectedfromthehealthdeliverywomenwithinformedcon—sent，inwhichthemononuclearcellswereisolated，andMSCswerefurth

5、erseparated，expandedtothe3passage．ThecharacteristicmarkerofMSCsofCD34，CD44andCD90wasidentifiedwiththeflowcytometryapplyingimmunofluorescenceway．Followingdifferentiationintroducedwith5-azafor4weeks，themyocardialmarkersofTroponinI，GATA4and8一MHCweredetectedbyimmunohis

6、tochem—istrystainingandRT—PCR，respectively．Results：TheMSCsinducingwith5-azashowedchangesoffi—broblast—likemorphologyandclonallyexpandedcharacteristics．Theimmunophenotypeofthesecellswasconsistentwiththatreportedfrombonemarrowmesenchymalstemcells．Themyocardialmarkers

7、ofTroponinI，GATA4andMHCwerepositivelydetectedbyimmunohistochemistrystainingandRT—PCR，respectively．Conclusion：OurdataindicatethatumbilicaIcordbloodcanbeservedasavaluableandconvenientSOUrCeofmesenchyma1stemcells．whichcandifferentiateintocardiomyocyte-likecell。andcanb

8、eusedasanalternativesourceinplaceofbonemarrow-derivedstemcells．[Keywords]Mesenchymalstemcells；Umbilicalcordblood；5-aza；Cardiomyocyte越来越多的证据表明，成人来