非小细胞肺癌外周血LUNX mRNA表达的临床意义-论文.pdf

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1、广西医学2014年5月第36卷第5期581tied,细胞肺癌外周血LUNXmRNA表达的临床意义▲池惠良林志潮伍硕允伍金华黄文海(广东省江门市中心医院1胸外科，2检验科，江门市529030，E-mail：shelleeli@126．com)【摘要】目的探讨非小细胞肺癌(NSCLC)患者外周血肺特异性x蛋白基因(LUNX)mRNA表达与血微转移的关系。方法选取9O例NSCLC(实验组)，其中I期31例，Ⅱ期32例，ⅢA期27例；肺良性疾病组20例，分别于术前、术中关胸时、术后第7天抽取外周血，RT—PCR法检测LUNXmRNA表达情况，并与健康体检者l

2、O例对照。结果健康对照组及肺良性疾病组均无LUNXmRNA表达。实验组术前、术中、术后LUNXmRNA表达阳性率分别为46．67％、65．56％及20．00％，术中LUNXmRNA表达阳性率高于术前(P<0．01)，术后低于术前(P

3、；肺特异性x蛋白基因；信使mRNA；微转移【中图分类号】R734．2【文献标识码】【文章编号】0253-4304(2014)05-0581—04DoI：10．11675／j．issn．0253-4304．2014．05．10ClinicalsignificanceofLUNXmRNAExpressioninPeripheralBloodofPafien~withNon-SmallCellLungCancerCHIHui—liang，LINZhi—chao，WUShuo-yun，删Jin—hua，HUANGWen—hai(1DepartmentofTh

4、oracicSu~ery，2ClinicalLaboratory，JiangmenCentralHospital，Jiangmen529030，China)【Abstract】ObjectiveToexploretherelationshipbetweentheexpressionoflung—specificXproteingene(LUNX)mRNAandmicrometastasisintheperipheralbloodofpatientswithnon—smallcelllungcancer(NSCLC)．MethodsNinetypati

5、entswithNSCLC(experimentalgroup)included31casesofstageI，32casesofstageIIand27casesofstagemA．Twentycasesofbenignpulmonarydisease(benignpulmonarydiseasegroup)and10healthycontrols(controlgroup)wereenrolledintothestudy．Theperipheralbl0odwascollectedfromthesubjectsinthebenignpulmona

6、rydiseasegroupandcontrolgroupbeforeoperation，justafteroperationand7daysafteroperation，respectively，tlleexpression~ofLUNXmRNAintheperipheralbloodwasdetectedbyRT—PCR，andacomparisonoftheresultswasconductedbetweentwogroups．ResultsNoneofLUNXmRNAwasdetectedinthecontrolgroupandbenignp

7、ulmonarydiseasegroup．ThepositiveratesofLUNXmRNAexpressionintheexperimentalgroupbeforeoperation，duringoperationand7daysafteroperationwere46．67％，65．56％and20．00％，respectively，thepositiverateofLUNXmRNAexpressionincreasedsignificantlyduringoperationincontrastwiththatbeforeoperation(

8、P<0．01)，thepositiverateofLUNXmRNAexpressiondecreasedsi