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时间:2020-06-03
《地塞米松对人颗粒脂肪裸鼠移植成活的影响.pdf》由会员上传分享,免费在线阅读,更多相关内容在应用文档-天天文库。
1、中国实用医药2014年4月第9卷第11期ChinaPraeMed,Apr2014,Vo1.9,No.1i·论著·地塞米松对人颗粒脂肪裸鼠移植成活的影响黄伟黄静王广宁王珏邸晶张玉【摘要】目的探讨地塞米松对自体脂肪颗粒移植成活率、脂肪纤维化及坏死程度的影响。方法将抽脂获得的颗粒脂肪组织经洗涤纯化后分为两组,实验组(A组)加入地塞米松,对照组(B组)不加地塞米松,只加入同等体积的生理盐水,将经过不同处理的两组脂肪分别移植于裸鼠背部皮下,3个月后取出裸鼠皮下的脂肪组织,测量其重量,计算其成活率,并进行HE染色
2、测定脂肪的囊腔数,评价其纤维化及坏死程度。结果湿重测定结果显示实验组脂肪颗粒注射移植的成活率f19.77士2.22)%高于对照组(12.80±1.73)%,两组间差异有统计学意义(P<0.01)。HE染色结果显示实验组脂肪的囊腔数(209.68±16.74)显著高于对照组(11.72±1.11),两组间差异有统计学意义(p3、neonthesurvivalrateofhumanfatparticleinnudemicetransplantationHUANGWei,HUANGJing,WANGGuang-ning,eta1.DepartmentofPlasticandReconstructiveSurgery,ZaozhuangMunicipalHospital,Zaozhuang277100,China【Abstract】ObjectiveToexploretheinfluenceofdexamethasoneonthe4、survivalrate,fatfibrosisandnecrosisdegreeoffatparticleautologous.MethodsFatparticleafterwashedfromhumanliposuctionwererandomlydividedintotwogroups:groupAandgroupB.Dexamethasonewereaddedasexperimentalgroup(groupA),andthesamevolumeofsalineaddedascontrolgr5、oup(groupB).TwoGroupsoffatparticlewererespectivelytransplantedonthebackofnudemice.Threemonthslater,tookoutthefatinsubcutaneoustissuetomeasuretheweightandcalculationsurvivalrateandHEstainingforthedeterminationoffatfibrosisandnecrosisdegree.ResultsThefatp6、articleauto-transplantationsurvivalrate(19.77±2.22)%inexperimentalgroupwashigherthanthatincontrolgroup(12.80±1.73)%thedifferencesbetweenthetwogroupsweresignificant(P<0.01).AfterHEstaining,thenumberofvesicles(209.68±16.74)inexperimentalgroupwashigherthan7、thatincontrolgroup(11.72±1.11),thedifferencesbetweenthetwogroupsweresignificant(P<0.01).ConclusionDexamethasonecanraisesurvivalrate0ffatparticleautologous.anddecreasefatfibrosisandnecrosisdegree.【Keywords】Dexamethasone;Fatparticleautologous;Survivalrate8、自体脂肪颗粒移植在整形外科领域应用已久,经常用于China),倒置相差显微镜(Olympus,Japan),超净工作台(苏州治疗面部软组织缺失导致的凹陷畸形或是面部美容手术,但安泰空气技术有限公司),冰箱(海尔,中国),5Oml离心管人体脂肪颗粒注射后吸收率较高,可达30%~40%,对于较大(FALCONFranklinLakesNT’U.S.A)。面积的面部凹陷,吸收率可达50%⋯,严重影响和限制了其1.3实验方法在整形美容领域的应用。为提高其成活率,
3、neonthesurvivalrateofhumanfatparticleinnudemicetransplantationHUANGWei,HUANGJing,WANGGuang-ning,eta1.DepartmentofPlasticandReconstructiveSurgery,ZaozhuangMunicipalHospital,Zaozhuang277100,China【Abstract】ObjectiveToexploretheinfluenceofdexamethasoneonthe
4、survivalrate,fatfibrosisandnecrosisdegreeoffatparticleautologous.MethodsFatparticleafterwashedfromhumanliposuctionwererandomlydividedintotwogroups:groupAandgroupB.Dexamethasonewereaddedasexperimentalgroup(groupA),andthesamevolumeofsalineaddedascontrolgr
5、oup(groupB).TwoGroupsoffatparticlewererespectivelytransplantedonthebackofnudemice.Threemonthslater,tookoutthefatinsubcutaneoustissuetomeasuretheweightandcalculationsurvivalrateandHEstainingforthedeterminationoffatfibrosisandnecrosisdegree.ResultsThefatp
6、articleauto-transplantationsurvivalrate(19.77±2.22)%inexperimentalgroupwashigherthanthatincontrolgroup(12.80±1.73)%thedifferencesbetweenthetwogroupsweresignificant(P<0.01).AfterHEstaining,thenumberofvesicles(209.68±16.74)inexperimentalgroupwashigherthan
7、thatincontrolgroup(11.72±1.11),thedifferencesbetweenthetwogroupsweresignificant(P<0.01).ConclusionDexamethasonecanraisesurvivalrate0ffatparticleautologous.anddecreasefatfibrosisandnecrosisdegree.【Keywords】Dexamethasone;Fatparticleautologous;Survivalrate
8、自体脂肪颗粒移植在整形外科领域应用已久,经常用于China),倒置相差显微镜(Olympus,Japan),超净工作台(苏州治疗面部软组织缺失导致的凹陷畸形或是面部美容手术,但安泰空气技术有限公司),冰箱(海尔,中国),5Oml离心管人体脂肪颗粒注射后吸收率较高,可达30%~40%,对于较大(FALCONFranklinLakesNT’U.S.A)。面积的面部凹陷,吸收率可达50%⋯,严重影响和限制了其1.3实验方法在整形美容领域的应用。为提高其成活率,
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