omega真菌DNA提取试剂盒说明书.pdf

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1、IntroductionContentsE.Z.N.A.™FungalDNAMiniprepKitsallowsfortherapidandreliableisolationofhigh-qualitytotalcellularDNAfromawidevarietyoffungalspeciesandIntroduction..................................................2tissues.Upto200mgofwettissue(orupto50mgdrytissue)canb

2、eprocessedinlessthan1hour.Thesystemcombinesthereversiblenucleic®Overview....................................................2acid-bindingpropertiesofOmega’sHiBindmatrixwiththespeedandversatilityofspincolumntechnologytoeliminatepolysaccharides,phenolicStorageandStabil

3、ity..........................................2compounds,andenzymeinhibitorsfromfungaltissuelysates.PurifiedDNAissuitableforPCR,restrictiondigestion,andhybridizationtechniques.ThereareKitContents.................................................3noorganicextractions,th

4、usreducingplasticwasteandhands-ontimetoallowmultiplesamplestobeprocessedinparallel.BeforeStarting...............................................3OverviewFungalDNAMiniprepProtocol...................................4IfusingtheE.Z.N.A.™FungalDNAMiniprepKitforthefirsttim

5、e,pleasereadthisbooklettobecomefamiliarwiththeprocedures.DryorfreshfungaltissueA.DrySpecimens.........................................4isdisruptedandthenlysedinaspeciallyformulatedbuffercontainingdetergent.Proteins,polysaccharides,andcellulardebrisaresubsequentlyB.Fr

6、esh/FrozenSpecimens................................6precipitated.ContaminantsarefurtherremovedbyisopropanolprecipitationofDNA.BindingconditionsarethenadjustedandthesampleisappliedtoanC.ShortProtocol.........................................9®HiBindDNAspin-column.Twora

7、pidwashstepsremovetracecontaminantssuchasresidualpolysaccharides,andpureDNAiselutedinwaterorlowionicVacuum/SpinProtocol........................................11strengthbuffer.PurifiedDNAcanbedirectlyusedindownstreamapplicationswithouttheneedforfurtherpurification.Tr

8、oubleshooting.............................................12StorageandStabilityAllcomponentsoftheE.Z.N.A.™FungalMiniprepKitarestabl