酵母基因克隆与表达载体（cloning and expression vector of yeast gene）

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1、酵母基因克隆与表达载体（Cloningandexpressionvectorofyeastgene）Two.Cloningandexpressionvectorofyeastgene(1)functionalframeworkcarrier--functionalstudyandcarriermodificationfordifferentuses1.Yeastintegratingplasmid(YIP)(1).Trpscreeningmarker(2).Urascreeningmarker(

2、3).Leuscreeningmarker(4).Hisscreeningmarker(5).G418screeningmarkerTheseplasmidscanbeintegratedintothegenomeofyeastcellsbyenzymatichydrolysisPurpose:theintegrationofA.genedeletionofB.genesubstitutedorstableexpressionelement(integration)andtype(knockin

3、)2.YeastCEN-ARSplasmid(YCP)(1).Trpscreeningmarker(2).Urascreeningmarker(3).Leuscreeningmarker(4).Hisscreeningmarker(5).G418screeningmarkerSuchplasmidscanautonomouslyreplicateinthecellandcontainnearlyonecopypercellbecauseofitsreplicationsequence.Usage

4、:becauseeachcellhasonlyonecopy,itavoidsthesurplusexpressionoftargetgene,soitisespeciallysuitableforthestudyofgenefunction3.Yeastepisomalplasmid(YEP)(1).Trpscreeningmarker(2).Urascreeningmarker(3).Leuscreeningmarker(4).Hisscreeningmarker(5).G418screen

5、ingmarkerTheseplasmidsarehighlyreplicatedincellswith200-400copiespercellUsage:usedfortheexpressionofforeigngenes(two)expressionvector1,GAL1promoterexpressionvector-galactoseinducedexpressionvector(1).Trpscreeningmarker(2).Urascreeningmarker(3).Leuscr

6、eeningmarker(4).Hisscreeningmarker(5).G418screeningmarker2,Cup1promoterexpressionvector-coppersulfateinducedexpressionvector(1).Trpscreeningmarker(2).Urascreeningmarker(3).Leuscreeningmarker(4).Hisscreeningmarker(5).G418screeningmarker3,ADHpromoterex

7、pressionvector-ADC1groupexpressionvector(1).Trpscreeningmarker(2).Urascreeningmarker(3).Leuscreeningmarker(4).Hisscreeningmarker(5).G418screeningmarkerLectureonyeastgenetictechnologyandhigherbiologicalgenefunction(Part1)expressionofforeigngenesinyeas

8、t2010-07-2411:51:11

9、classification:

10、labelmethodandyeastgenetics:yeastculturemediumselectiondefectofdropoutexpressionplasmid

11、report

12、namesubscriptionAuthor:PanYitechnicaldepartmentmodelbiologygroupZhuLeinuoBuddingyeast(buddingyeast,Saccharomycescerevis