大鼠门脉高压症脾次全切除术对部分免疫功能的影响

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1、大鼠门脉高压症脾次全切除术对部分免疫功能的影响作者：刘程伟张雪松王树卿罗维张强【摘要】目的：通过门脉高压症（PHT）脾次全切除术后细胞免疫和体液免疫的变化，初步评价脾次全切除术在门脉高压症中的作用。方法：建立肝硬化PHT大鼠疾病模型。实验分5组，每组10只。正常对照组（NCG），实验对照组（ECG），全脾切除组（TSG），保留正常脾脏50％的脾部分切除组（R50％NSG），保留正常脾脏大小的脾部分切除组（RNSG）。术后4周对各组进行血常规、免疫球蛋白、T淋巴细胞亚群的测定及病理学检查。结果：术后4周R50％NSG组与RNSG组与NCG组和ECG组血常规、免疫球蛋白和T淋巴细胞亚群水平

2、差异无统计学意义。ECG光镜下见假小叶，再生的肝细胞结节肝细胞索排列紊乱等肝硬化表现脾包膜增厚，脾实质充血严重。脾内纤维严重增生。R50％NSG和RNSG光镜下见肝纤维化程度减轻，脾包膜变薄，脾实质轻度充血。结论：PHT残余脾脏具有一定的生理功能；保留部分脾脏的脾部分切除术在一定程度上能够维持机体的体液免疫和细胞免疫。【关键词】脾次全切除术・门静脉高压症，门静脉・免疫功能Theinfluenceofsubtotalsplenectomyonpartial9immunlogicalfunctioninratswithporoalhypertension【ABS

3、TRACT】Objective:Todetectthechangeofcellimmunityandbodyfluidimmunityaftersubtotalsplenectomyinportalhypertensionofrat,andpreliminarilyevaluatetheroleofsubtotalsplenectomyinportalhypertension.Methods:Weestablishedtheratmodelofhepaticcirrhosisportalhypertension.Ratsweredividedtofivegroups:normalcon

4、trolgroup(NCG);experimentalcontrolgroup(ECG),totalsplenectomygroup(TSG),retainfiftypercentsizeofnormalspleengroup(R50%NSG),retainthesamesizeofnormalspleengroup(RNSG).MeasuredtheBloodroutine,Immunoglobulin,TIymphocytesubgroupandstainedwithhematoxylinandeosin(HE)at4weeksaftersurgery.Results:Therew

5、erenotsignificantdifferentbetweenRNSGandNCGinBloodroutine,Immunoglobulin,TIymphocytesubgroupat4weeksafteroperation.ECGcouldbeseenbylightmicroscopethatthetissueofnormalhepaticlobulewasdestructed,andappearedpseudolobule.Disarrangementofregeneratedhepaticcellnodusandlivercellcord,fattydegenerationi

6、nhepaticcell,thickeningofspleencapsuleandseriouscongestionofspleenparenchyma,atrophyofwhitepulpandshrinkingof9follicleandgerminalcenterR50%NSGandRNSGcouldbeseenthathepaticfibrosis,spleencapsulethicheningandcongesitionofsplenicparenchymatoalessextent.Conclusion:Remainingspleenofportalhypertension

7、causedbyhepaticcirrhosishasphysiologyfunctions.Insomedegree,splenectomyofretainingthesamesizeofnormalspleencouldmaintainbodyfluidimmunityandcellimmunityoforganism.【KEYWORDS】Subtotalsplenectomy・Portalhypertensionӥ