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1、RP多糖体内抗鸭乙型肝炎病毒实验研宄何云鹏陶慧慧刘群红（安徽理工大学医学院安徽淮南232001）【摘要】目的观察RP多糖在体内对DHBV的抑制作用。方法体内实验以先天感染DHBV的雏鸭为动物模型,将雏鸭（10只/组）随机分为5组（RP多糖高、中、低3个剂量组、3-TC组、生理盐水对照组），均以20mg/kg/d灌胃给药10天。给药前1天、给药后5天、10天及停药后3天，均采用斑点杂交法观察血清中DHBV-DNA表达。结果各剂量组RP多糖对DHBV均有相应的抑制作用。其中给药后第5天，高、中剂量组血清DHBV-DNA为0.77&plus
2、mn;0.27、0.69±0.15,第10天为0.57±0.11、0.49±0.19,与生理盐水对照组（1.32±0.56、1.24±0.53）相比明显降低。给药后第5天，RP多糖高、中剂量组对DHBV-DNA的抑制率为25.16%、25.35%,第10天为45.94%、48.28%,与生理盐水对照组对比显著提高。结论RP多糖可降低血DHBV-DNA含量，对DHBV有抑制作用。【关键词】菲律宾蛤仔多糖鸭乙肝动物模型乙型肝炎【中图分类号】R392-3【文献标识码】A【
3、文章编号】2095-1752（2014）04-0041-02ExperimentalStudyofRuditapesphilippinarumPolysaccharideonAnti-DHBVinVivoHeYunpengTaoHuihuiLiuQunhong(SchoolofMedicine，AnhuiUniversityofScience&Technology，Huainan，Anhui232001，China)【Abstract】Objective:Toobservethein-vivoinhibitoryeffectofRPp
4、olysaccharideonDHBV.Methods:1-dayoldBeijingducklingswithcongenitalinfectionofDHBVwereusedastheanimalmodelandwererandomlydividedintofivegroups(Namely,RPpolysaccharidehighdosegroup,mediumdosegroup,lowdosegroup,Lamivudinegroupandnormalsalinegroup).Eachgroupofducksweregiven
5、thedrugwiththedoses20mg/kg/dfor10days.TheexpressionofDHBV-DNAwasdetectedbydot-blothybridizationtestldaybeforetreatment,fivedaysandtendaysduringtreatment,and3daysafterdrugwithdrawal,respectively.Results:IneachgroupoftheRPpolysaccharidehascorrespondinginhibitiononDHBV.Aft
6、erbeingmedicatedwithRPpolysaccharideforfivedays,inRPpolysaccharidehighdosegroup,mediumdosegroup,SerumDHBV-DNAwas0.77±0.27,0.69±0.15;whilefortendays,0.57±0.11,0.49±0.19,respectively,whichwasobviouslylowercomparedwiththoseinnormalsalinegroup(1.
7、28±0.54,1.20±0.5l).TheinhibitionratesofDHBV-DNAinRPpolysaccharidehighdosegroup,mediumdosegroupwere25.16%,25.35%respectively5daysaftertreatment,while45.94%,48.28%;respectively10daysaftertreatment,indicatingremarkableimprovementcomparedwiththecontrolgroup.Co
8、nclusion:RPpolysaccharidecanprotectduckliveragainstinflammationbytheinhibitionofDHBV.[Keywords]Ruditapesphilip
