软骨细胞复合β-tcp生物陶瓷构建组织工程软骨应用研究

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1、口growthandproliferatiOil，andpassedintothe109arithmicphaseafterAfterabriefplateauperiod．The1st一4thgenerationcellshavenOsignificantchangesinthegrowthcurve．The5thgenerationofcellgrowthabilitystartedtodim[aishandthe6theellsproliferationwasmarkedlyreduced．Passageeellsofauriclecartilagecel

2、lsshowingstronggrowthandproliferation，andpassedintothelogarithmicphaseinthevaccinationday．The1st～4thgenerationeellshavenosignificantchangesinthegrowthcurveThe5thgenerationofcellgrowthabilitystartedtodiminishandthe6thcellSproliferationwasmarkedlyreducedParticularlythe2ndgenerationofca

3、rtilagecellproliferationisthestrongest．andthereiSnOincubationperiodinthegrowthcurve(3)Porousstructureof8一TCPporousbioceramiccompositematerialscanbebettercombinedwithchondrocytesforcartilagetissueengineeringandcultivation，andcanmaintainthechondrocytephenotypeforalongtime．Themicro—stru

4、ctureofintercnnectiondiameteri00umandporediameter530600ummoreadaptedtheauriclecartilagecellculture．CartilagecellSintheenvironmentofporousmicro—structurecanbemaintainedthephenotypeto4thgeneration．(4)UsingBTCPbioceramicscompositecartilageeellScomparedwiththecontrolgrouptoconfirmethatCh

5、ondrocytescombinedwithB—TCPbioceramicscompositestructureoftissueengineeredcartilagecanrepairthearticularcartilagedefectsmodel．AuriclecartilageceilsandArticularcartilageceIlscombinedⅣiththemicro—structureofintercnneetiondiameter100u口andporediameter530—600pmcarlbettermaintainthecellphe

6、notypeandextracellularmattixupdatespeedWeusedD’DriSCO】lmethodtoaccordancethecart【lagemorphologyscore．Thesethreegroupsofcontrolgroup，articularcartilageeellsgroupandauric]echondrocytesgroupscorefollowedbyincreased．TheSCDreiscontrolgroup525_+888．articulareartilageceilsgroup18．37±1．40．au

7、riclechondrocytesgroup1912土1．45ArticularcartilageeellSgroupandauriclecartilageceIIsgroupbothhasstatisticallysignificantdifferencewithcontrolgroup．Thereare[10statisticaIlySignificantdiCf'erencebetweenarticuIarcartilageeellSgroupandauriclecartilagecellSgroup，ConclUSiOil：l，Auriclecarti1

8、agecelIdiSSO