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1、生理学报,Dec.2000,52(6):445～449 445 ActaPhysiologicaSinica研究论文17β2雌二醇抑制雄性大鼠颈动脉窦压力感受器反射王 升,范振中,何瑞荣3(河北医科大学基础医学研究所生理室,石家庄050017)摘 要: 采用隔离灌流麻醉雄性大鼠颈动脉窦技术,观察了17β2雌二醇(E2)对颈动脉窦压力感受器反射的影响。结果如下:(1)以E2(10μmol/L)隔离灌流颈动脉窦区时,压力感受器机能曲线向右上方移位,曲线最大斜率(peakslope,PS)由0149±0103降至0125±0101(P<0101),反射性血
2、压下降幅度(reflexdecrease,RD)由7137±0142kPa降至3149±0120kPa(P<01001),阈压(thresholdpressure,TP)和饱和压(saturationpressure,SP)分别由9152±0168kPa和24153±0148kPa增至1313±0111kPa(P<01001)和27152±0120kPa(P<0101),其中PS、RD、TP和SP呈明显的剂量依赖性;(2)用雌激素受体阻断剂tamoxifen(1、5、10、30μmol/L)预处理后,不能阻断E2对压力感受器反射的抑制作用;(3)预先
3、灌流NO合酶阻断剂(L2NAME,100μmol/L),可完全消除E2(10μmol/L)对压力感受器反射的抑制效应。以上结果表明,17β2雌二醇可通过非基因组机制抑制大鼠颈动脉窦压力感受器反射,其效应系E2引起血管内皮细胞释放NO所致。关键词:雌激素;17β2雌二醇;它莫西芬;压力感受器反射;平均动脉压;非基因组效应学科分类号:Q46317β2estradiolinhibitscarotidsinusbaroreflexinmaleratsWANGSheng,FANZhen2Zhong,HERui2Rong3(DepartmentofPhysiol
4、ogy,InstituteofBasicMedicine,HebeiMedicalUniversity,Shijiazhuang050017)Abstract: Byperfusingisolatedcarotidsinus,theeffectof17β2estradiol(E2)oncarotidsinusbaroreflexwasobservedinanesthetizedmalerats.Theresultsobtainedareasfollows.(1)ByperfusingwithE2(10μmol/L),thefunctionalcurv
5、eofbaroreflexwasshiftedtotherightandupward,withapeakslope(PS)de2creasefrom0149±0103to0125±0101(P<0101)andareflexdecreaseinmeanarterialpressure(reflexdecrease,RD)from7137±0142kPato3149±0120kPa(P<01001),whilethethresholdpressure(TP)andsaturationpressure(SP)weresignificantlyincrea
6、sedfrom9152±0168kPato1313±0111kPa(P<01001)and24153±0148kPato27152±0120kPa(P<0101)respectively.Amongthefunctionalparame2tersofcarotidbaroreflex,thechangesofRD,PS,TPandSPweredose2dependent.(2)Pretreatmentwithdif2ferentdosesoftamoxifen(1,5,10,30μmol/L),aninhibitorofestrogenrecepto
7、r,didnotblocktheeffectofE2oncarotidbaroreflex.(3)PreperfusionwithaninhibitorofNOsynthaseL2NAME(100μmol/L)couldcompletelyabolishtheeffectofE2oncarotidbaroreflex.ItisconcludedthattheinhibitoryeffectofE2oncarotidsinusbaroreflexmaybemediatedbyNOreleasefromendothelialcells,butnotbya
8、genomicmecha2nism.Keywords:17β2estradiol;baroreflex;me
