反相高效液相法测定温胃舒片中补骨脂素和异补骨脂素的含量论文

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1、反相高效液相法测定温胃舒片中补骨脂素和异补骨脂素的含量论文蔡皓，张科卫，李伟，崔小兵【摘要】目的建立一种测定温胃舒片中补骨脂素和异补骨脂素含量的反相高效液相法。方法采用KromasilC18柱，以甲醇水(55∶45)为流动相，紫外检测波长246nm，流速1ml·min-1。结果在所使用的色谱条件下，补骨脂素和异补骨脂素出峰位置未见干扰.freell-1和5.02～30.12μg·ml-1浓度范围内线性关系良好(r=0.9999)。温胃舒片中补骨脂素和异补骨脂素的平均加样回收率分别为97.64%和99.42%，相对标准偏差分别为1.16%(n=6)和0.86%(n=6)。结论该

2、法可信度高，操作简便，重现性好，可用于温胃舒片生产上的质量控制。【关键词】温胃舒片；补骨脂素；异补骨脂素；反相高效液相法；定量分析DeterminationoftheContentsofPsoralenandIsopsoralenContainedinacrocephalae,FructusCrataegi,FructusMume,FructusAmomi,.freelCitriReticulataeandFructusPsoraleaebythemethodofboilingingandnourishingstomach，promoting"qi"circulationtor

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6、P.R.China)andtheirpuritiesRP-HPLC.edicinalMaterials,2003,26(3)：185.［3］LIANGShenginationofeffectivepoundsinPsoraleacorylifoliabyGC［J］.ChinesePharmaceuticalJournal,1996,31(7)：394.［6］XingIHeming,edicalScience,2003,5(2)：85.［8］XUYong,LILedao,XIONGZhili,HUANGYuyin,GUOXingjie.QuantitativeDeterminat

7、ionofPsoralenandIsopsoraleninPsoraleaecorylifaliabyHighPerformanceLiquidChromatography［J］.ChineseJournalofChromatography,2003,21(4)：385.